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Lasp1 (NM_010688) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203460
Lasp1 (Myc-DDK-tagged) - Mouse LIM and SH3 protein 1 (Lasp1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lasp1
Synonyms AA408629; Def-4; SH3P6; Tg(Col1a1-lacZ)1Ngma
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203460 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCCTAACTGTGCCCGGTGCGGCAAGATCGTGTACCCCACGGAGAAGGTGAACTGTCTGGATAAGT
ACTGGCATAAAGCATGCTTTCACTGCGAGACCTGCAAGATGACCCTGAACATGAAGAACTACAAGGGTTA
TGAGAAGAAGCCTTACTGCAATGCACACTATCCCAAGCAGTCCTTCACCATGGTGGCCGACACTCCGGAA
AATCTCCGCCTCAAGCAACAGAGCGAGCTGCAGAGTCAGGTGCGCTACAAGGAGGAATTTGAGAAGAATA
AGGGCAAAGGTTTCAGCGTGGTGGCAGACACGCCTGAGCTGCAGAGAATCAAGAAGACCCAGGACCAGAT
CAGCAATATCAAATACCATGAGGAGTTTGAGAAGAGCCGCATGGGGCCCAGTGGAGGAGAAGGGGTGGAA
CCAGAGCGCCGAGAAGCCCAGGACAGCAGCAGCTACCGGAGGCCCACAGAGCAGCAGCAGCCGCAGCCTC
ACCATATCCCGACCAGTGCCCCCGTGTACCAGCAGCCCCAGCAGCAGCAGATGACCTCGTCCTATGGTGG
GTACAAGGAGCCAGCAGCCCCTGTCTCCATACAGCGCAGTGCCCCAGGTGGCGGTGGGAAACGGTACCGT
GCAGTGTATGACTACAGCGCTGCCGACGAGGACGAGGTCTCCTTCCAGGATGGGGACACCATCGTCAATG
TGCAGCAGATCGATGACGGCTGGATGTACGGGACCGTAGAGCGCACCGGTGACACGGGGATGCTGCCAGC
CAACTACGTGGAGGCCATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203460 protein sequence
Red=Cloning site Green=Tags(s)

MNPNCARCGKIVYPTEKVNCLDKYWHKACFHCETCKMTLNMKNYKGYEKKPYCNAHYPKQSFTMVADTPE
NLRLKQQSELQSQVRYKEEFEKNKGKGFSVVADTPELQRIKKTQDQISNIKYHEEFEKSRMGPSGGEGVE
PERREAQDSSSYRRPTEQQQPQPHHIPTSAPVYQQPQQQQMTSSYGGYKEPAAPVSIQRSAPGGGGKRYR
AVYDYSAADEDEVSFQDGDTIVNVQQIDDGWMYGTVERTGDTGMLPANYVEAI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_010688
ORF Size 789 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_010688.5
RefSeq Size 3433 bp
RefSeq ORF 792 bp
Locus ID 16796
UniProt ID Q61792
Cytogenetics 11 61.1 cM
MW 30 kDa
Summary Plays an important role in the regulation of dynamic actin-based, cytoskeletal activities. Agonist-dependent changes in LASP1 phosphorylation may also serve to regulate actin-associated ion transport activities, not only in the parietal cell but also in certain other F-actin-rich secretory epithelial cell types (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_010688" in other vectors (4)
SKU Description Size Price
MC207314 Lasp1 (untagged) - Mouse LIM and SH3 protein 1 (Lasp1), (10ug) 10 ug
$330.00
MG203460 Lasp1 (tGFP-tagged) - Mouse LIM and SH3 protein 1 (Lasp1) 10 ug
$500.00
MR203460L3 Lenti ORF clone of Lasp1 (Myc-DDK-tagged) - Mouse LIM and SH3 protein 1 (Lasp1) 10 ug
$600.00
MR203460L4 Lenti ORF clone of Lasp1 (mGFP-tagged) - Mouse LIM and SH3 protein 1 (Lasp1) 10 ug
$600.00

Plasmid Map for MR203460

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203460
Circular map for MR203460

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