Mrpl10 (NM_026154) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203437
Mrpl10 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L10 (Mrpl10), nuclear gene encoding mitochondrial protein
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrpl10
Synonyms 0610040E02Rik; C78440; MRP-L8; Rpml8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203437 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGGCGGTGGCCGGGATCCTCCGTGGAGGTCTCCCGCCCCGGGCGGCTTGGCTGCCCACCCTCC
AGACTGTGCGCCATGGCTCCAAGGCTGTGACCCGCCACTGGCGAGTCATGCACTTCCAGCGGCAGAAGCT
GATGGCTATAACTGAGTATATTCCTCCCAAACCTGCCATCAACCCGAGATGCCTGCCACCTCCTCCCAAG
CCCCCCAAGGAGGAGTCAGGCCTCGTCCGGCTCCTGCGTCAAGATATAGTAGCAGTTTTTCGGGACAACC
GAATGATAGCTGTCTGCCAGAACGTGGCCCTGAGTGCAGAGGACAAGCTTCTCCTGAGACACCAGCTGCG
GAAACACAAGATCTTCATAAAAGTCTTCCCCAGCCAGGTCCTAAAGCCTTTTCTAGAAAATTCCAAATAC
CGAAACCTGCTACCCCTTTTTGTCGGACACAACCTGCTGCTGGTCAGTGAGGAGCCCAAGGTCAAGGAAA
TGGTGCGGGTCTTAAAGAGCGTTCCTTTCCTGCCGCTGCTGGGTGGCTGCGTTGATGACACCATCCTCAG
CAGGCAGGGACTCGTAGACTATGCCAAGCTGCCCAGCCTGGATCAGCTACAGGGGCAGCTGGTAGGAGGG
CTTACTCACCTCATGGCCCAGACCCGCTACCTGCTTCAGCACCAGCCTGTCCAGCTGACTTCCCTGTTGG
ATCAGTATGTCAAAGAGCAAAACGAGGGAGATTGTGCCACGTCAGCCAATGAGAAGCTACATCCTCCCGA
TCCTGCTCCAGATGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203437 protein sequence
Red=Cloning site Green=Tags(s)

MAAAVAGILRGGLPPRAAWLPTLQTVRHGSKAVTRHWRVMHFQRQKLMAITEYIPPKPAINPRCLPPPPK
PPKEESGLVRLLRQDIVAVFRDNRMIAVCQNVALSAEDKLLLRHQLRKHKIFIKVFPSQVLKPFLENSKY
RNLLPLFVGHNLLLVSEEPKVKEMVRVLKSVPFLPLLGGCVDDTILSRQGLVDYAKLPSLDQLQGQLVGG
LTHLMAQTRYLLQHQPVQLTSLLDQYVKEQNEGDCATSANEKLHPPDPAPDA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026154
ORF Size 786 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026154.3
RefSeq Size 1682 bp
RefSeq ORF 789 bp
Locus ID 107732
UniProt ID Q3TBW2
Cytogenetics 11 D
MW 29.4 kDa
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Documents
Resources
"NM_026154" in other vectors (4)
SKU Description Size Price
MC201273 Mrpl10 (untagged) - Mouse mitochondrial ribosomal protein L10 (Mrpl10), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG203437 Mrpl10 (tGFP-tagged) - Mouse mitochondrial ribosomal protein L10 (Mrpl10) 10 ug
$500.00
MR203437L3 Lenti ORF clone of Mrpl10 (Myc-DDK-tagged) - Mouse mitochondrial ribosomal protein L10 (Mrpl10), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR203437L4 Lenti ORF clone of Mrpl10 (mGFP-tagged) - Mouse mitochondrial ribosomal protein L10 (Mrpl10), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR203437

Download sequence for

Cloning scheme for MR203437

Circular map for MR203437

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