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Trh (NM_009426) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203300
Trh (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone (Trh)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Trh
Synonyms Pro-TRH; Trf
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203300 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGGACCTTGGCTGATGATGGCTCTGGCTTTGATCTTCGTGCTAACTGGTATCCCCAAATCCTGCG
CCTTGCTGGAAGCAGCCCAGGAGGAAGGTGCTGTGACTCCTGACCTTCCAGGCCTGGAGAAAGTCCAGGT
CCGGCCAGAACGTCGATTCTTGAGGAAAGACCTCCAGCGTGTGCGAGGGGACCTTGGTGCTGCCTTAGAT
TCCTGGATCACAAAACGCCAGCATCCGGGCAAAAGGGAGGAGAAGTTGGAAGACGAAGCCGAAGAGAGGG
GAGATTTGGGAGAAGTGGGAGCCTGGAGACCCCACAAACGACAGCACCCTGGCCGACGTGCCAACCAAGA
CAAGGATTCATGGTCAGATGAGGGGGACAGTGACTGGCTGCCCCCGTCCTGGCTACCAGATTTCTTTCTG
GATTCCTGGTTCTCAGATGCCCCCCAAGTCAAGCGGCAGCACCCTGGCAGGCGATCCTTCCCCTGGATGG
AGTCTGATGTCACCAAGAGGCAGCATCCAGGCCGGAGGTTCATAGATCCTGAGCTCCAAAGAAGCTGGGA
AGAAACAGAGGGAGAGGAGGGTGGCTTAATGCCTGAGAAACGTCAGCATCCTGGCAAAAGGGCAGTGGGT
CACCCTTGTGGGCCCCAGGGGATTTGTGGCCAAACAGGCCTGCTCCAGCTTCTAGGTGACCTGAGTAGGG
GCCAGGAGACCCTGGCAAAGCAAACCCCACAACTGGAAGCCTGGGTCAGGGAACCTCTGGAGGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203300 protein sequence
Red=Cloning site Green=Tags(s)

MQGPWLMMALALIFVLTGIPKSCALLEAAQEEGAVTPDLPGLEKVQVRPERRFLRKDLQRVRGDLGAALD
SWITKRQHPGKREEKLEDEAEERGDLGEVGAWRPHKRQHPGRRANQDKDSWSDEGDSDWLPPSWLPDFFL
DSWFSDAPQVKRQHPGRRSFPWMESDVTKRQHPGRRFIDPELQRSWEETEGEEGGLMPEKRQHPGKRAVG
HPCGPQGICGQTGLLQLLGDLSRGQETLAKQTPQLEAWVREPLEE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009426
ORF Size 765 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009426.1, NM_009426.2, NM_009426.3, NP_033452.2
RefSeq Size 1400 bp
RefSeq ORF 771 bp
Locus ID 22044
UniProt ID Q62361
Cytogenetics 6 41.03 cM
MW 29.1 kDa
Summary This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The mouse proprotein contains five thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Disruption of this gene results in hypothyroidism, elevated thyroid-stimulating hormone levels, and hyperglycemia. provided by RefSeq, Apr 2013
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Documents
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"NM_009426" in other vectors (4)
SKU Description Size Price
MC202032 Trh (untagged) - Mouse thyrotropin releasing hormone (Trh), (10ug) 10 ug
$300.00
MG203300 Trh (tGFP-tagged) - Mouse thyrotropin releasing hormone (Trh) 10 ug
$500.00
MR203300L3 Lenti ORF clone of Trh (Myc-DDK-tagged) - Mouse thyrotropin releasing hormone (Trh) 10 ug
$600.00
MR203300L4 Lenti ORF clone of Trh (mGFP-tagged) - Mouse thyrotropin releasing hormone (Trh) 10 ug
$600.00

Plasmid Map for MR203300

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203300
Circular map for MR203300

file-alt Citations

Differential regulation of thyrotropin-releasing hormone mRNA expression in the paraventricular nucleus and dorsomedial hypothalamus in OLETF rats
Zhang, Zhang, Bi et al
Neurosci. Lett. (2019) 703, 79-85 DOI: 10.1016/j.neulet.2019.03.030
AI Summary: OriGene’s full-length mouse preproTrh cDNA was used in this study to generate probes for in situ hybridization, enabling the researchers to measure Trh mRNA expression in specific hypothalamic regions.
Snippet: caudal to bregma) were selected [21,22]. 2.5. In situ hybridization (ISH) determination. The full length of mouse preproTrh cDNA (OriGene Technologies, Inc) was sub-cloned into pGEM-11Zf(+). As previously described [21], 35 S
Showing 1-1 of 1 papers.
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