Tspan3 (NM_019793) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR203238
Tspan3 (Myc-DDK-tagged) - Mouse tetraspanin 3 (Tspan3)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tspan3
Synonyms 1700055K04Rik; TM4-A; Tm4sf8
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203238 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCCAATGCGGCATCACCTCCTCTAAGACCGTGCTGGTCTTCCTCAACCTCATCTTCTGGGGGGCAG
CTGGCATCCTCTGCTACGTGGGAGCCTATGTCTTCATCACCTATGACGACTATGACCACTTCTTCGAGGA
TGTGTACACGCTCTTCCCTGCCGTGGTGATCATAGCTGTAGGAGCCTTGCTTTTCATCATTGGCCTGATC
GGCTGCTGTGCTACAATCCGGGAGAGCCGCTGTGGACTTGCCACGTTTGTCTTCATCCTGCTCTTGGTTT
TCGTCACAGAAGTGGTTGTTGTGGTTTTGGGATACGTGTACAGAGCAAAGGTGGAGAACGAGGTTGATCG
CAGCATTCAGAAAGTGTATAAGACCTACAACGGGACCAACTCCGACGCTGCCAGCCGTGCTATTGACTAT
GTGCAGAGACAGTTGCACTGTTGTCGAATTCATAACTATTCAGACTGGGAAAATACAGATTGGTTCAAAG
AAACAAAAAACCAGAGTGTCCCTCTTAGCTGCTGCAGAGAGACTGCCAAAAGCTGTAACGGCAGCCTGGC
CAACCCCTCTGACCTCTACGCCGAGGGCTGTGAGGCTCTTGTTGTGAAGAAGCTACAAGAAATCTTGATG
CATGTTATCTGGGCAGCGTTGGCCTTTGCAGCTATTCAGCTGCTGGGCATGCTGTGCGCATGCATCGTGT
TGTGCAGACGGAGTAGAGATCCTGCCTATGAGCTCCTCATCACTGGCGGAACCTACGCG


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR203238 protein sequence
Red=Cloning site Green=Tags(s)

MGQCGITSSKTVLVFLNLIFWGAAGILCYVGAYVFITYDDYDHFFEDVYTLFPAVVIIAVGALLFIIGLI
GCCATIRESRCGLATFVFILLLVFVTEVVVVVLGYVYRAKVENEVDRSIQKVYKTYNGTNSDAASRAIDY
VQRQLHCCRIHNYSDWENTDWFKETKNQSVPLSCCRETAKSCNGSLANPSDLYAEGCEALVVKKLQEILM
HVIWAALAFAAIQLLGMLCACIVLCRRSRDPAYELLITGGTYA

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_019793
ORF Size 759 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019793.2, NP_062767.2
RefSeq Size 1747 bp
RefSeq ORF 762 bp
Locus ID 56434
UniProt ID Q9QY33
Cytogenetics 9 B
MW 28.1 kDa
Summary Regulates the proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019793" in other vectors (3)
SKU Description Size Price
MG203238 Tspan3 (tGFP-tagged) - Mouse tetraspanin 3 (Tspan3) 10 ug
$500.00
MR203238L3 Lenti ORF clone of Tspan3 (Myc-DDK-tagged) - Mouse tetraspanin 3 (Tspan3) 10 ug
$600.00
MR203238L4 Lenti ORF clone of Tspan3 (mGFP-tagged) - Mouse tetraspanin 3 (Tspan3) 10 ug
$600.00

Plasmid Map for MR203238

Download sequence for

Cloning scheme for MR203238

Circular map for MR203238

file-alt Citations

Tetraspanin-3 is an organizer of the multi-subunit Nogo-A signaling complex
Thiede-Stan, Tews, Albrecht et al
J. Cell. Sci. (2015)
Cited by 23
AI Summary: OriGene products, including TrueORF cDNA plasmids for TSPAN3 (MR203238) and TSAPN7 (MR203045) as well as anti-DDK antibodies (TA50011), were used for gene expression and protein detection to investigate the role of tetraspanin-3 in organizing the Nogo-A signaling complex.
Snippet: TrueORF cDNA plasmids (TSPAN3-mycDDK or TSAPN7-mycDDK) (OriGene MR203238 and OriGene MR203045) and/or S1PR2-GFP plasmid (cloned by Michael Arzt, Brain ... anti-DDK antibodies (OriGene TA50011, 2.5 μg/ 500 μl protein lysate) or isotype control ... Cite SaveSaving
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Showing 1-1 of 1 papers.
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