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Taf9b (BC066223) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203166
Taf9b (Myc-DDK-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Taf9b
Synonyms BC066223; Taf9l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203166 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCGGCCAAGATGGCGCCTATCAAGAACGCTCCGAGAGATGCCTTGGTGATGGCACAGATCCTGA
AGGATATGGGAACCACAGAGTATGAGCCAAGGGTTATAAATCAAATGTTGGAATTTGCTTTCCGATATGT
GACTACAATTCTGGACGATGCAAAGATTTATTCGAGTCATGCTAAGAAACCCACTGTTGATGCCGATGAC
GTGAGACTGGCCATCCAGTGTCGGGCTGACCAGTCCTTTACCTCTCCTCCCCCAAGAGATTTTTTACTAG
ATATTGCAAGGCAGAAGAATCAAACCCCTTTGCCGCTGATTAAGCCATATGCAGGACCCAGACTGCCACC
TGATAGATACTGCTTAACAGCTCCAAACTATAGGCTGAAGTCCTTAGTTAAAAAGGGACCTAACCAAGGA
AGACTAGTGCCTAGATTAAGTGCGGTCAGTAGTAGACCAACTACTCCTCCTGTAGCACCCCCACAAGCAG
TATCTGGCCCAAATAAGGCTGCAACTCCGGTGTCAGTGACAAGCCAAAGATTTGCTGTGCAGATTCCACC
TTCTCAGTCCACACCTGCCAAGCCAGCTCCTGCAGCAACTGCAGTCCAAAATGTTCTGATTAATCCTTCA
ATGATTGGGCCCAAAAATATTCTCATTACCACCAGCATGGTTTCCTCACAGAACACAGCCACCGACTCAA
ACCCACTGAAGAGGAAGCATGATGACGACGATGACAATGACACCATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203166 protein sequence
Red=Cloning site Green=Tags(s)

MEPAKMAPIKNAPRDALVMAQILKDMGTTEYEPRVINQMLEFAFRYVTTILDDAKIYSSHAKKPTVDADD
VRLAIQCRADQSFTSPPPRDFLLDIARQKNQTPLPLIKPYAGPRLPPDRYCLTAPNYRLKSLVKKGPNQG
RLVPRLSAVSSRPTTPPVAPPQAVSGPNKAATPVSVTSQRFAVQIPPSQSTPAKPAPAATAVQNVLINPS
MIGPKNILITTSMVSSQNTATDSNPLKRKHDDDDDNDTM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC066223
ORF Size 747 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC066223, AAH66223
RefSeq Size 2393 bp
RefSeq ORF 749 bp
Locus ID 407786
Cytogenetics X D
MW 27.2 kDa
Summary Essential for cell viability. TAF9 and TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. May have a role in gene regulation associated with apoptosis. TAFs are components of the transcription factor IID (TFIID) complex, the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TFIID or TFTC are essential for the regulation of RNA polymerase II-mediated transcription (By similarity).UniProtKB/Swiss-Prot Function
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"BC066223" in other vectors (3)
SKU Description Size Price
MG203166 Taf9b (tGFP-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785 10 ug
$500.00
MR203166L3 Lenti ORF clone of Taf9b (Myc-DDK-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785 10 ug
$600.00
MR203166L4 Lenti ORF clone of Taf9b (mGFP-tagged) - Mouse TAF9B RNA polymerase II, TATA box binding protein (TBP)-associated factor (cDNA clone MGC:76785 10 ug
$600.00

Plasmid Map for MR203166

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR203166
Circular map for MR203166

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