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Tmem33 (BC005562) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203124
Tmem33 (Myc-DDK-tagged) - Mouse transmembrane protein 33 (cDNA clone MGC:11715 IMAGE:3965864)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem33
Synonyms 1110006G02Rik; 1600019D15Rik; 2410089A21Rik; 2700052H22Rik; 5430406L04Rik; AA388285; AI314185; AI426615; db83
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203124 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGATACGACCCCGAACGGCCCCCAAGGGGCGGGCGCTGTGCAATTCATGATGACCAATAAATTGG
ACACAGCAATGTGGCTTTCTCGCCTGTTCACAGTTTATTGCTCCGCTCTGTTCGTTCTGCCTCTTCTTGG
GTTGCATGAAGCAGCGAGCTTTTACCAGCGTGCTTTGCTGGCCAATGCTCTGACCAGCGCTCTGAGGCTG
CATCAGAGATTACCTCACTTCCAGTTGAGCAGAGCGTTCCTGGCTCAGGCCTTGTTAGAGGACAGCTGCC
ACTACCTGCTGTATTCACTCATCTTCGTCAACTCCTACCCTGTCACAATGAGTATTTTTCCAGTCTTGTT
ATTTTCTTTGCTCCATGCTGCCACATACACTAAGAAGGTCCTGGACGCAAAGGGTTCAAATAGTTTACCT
CTGTTGAGATCATTCTTGGATAAATTAAGTACTAACCAACAAAATATTCTGAAATTCATCGCTTGTAATG
AAATATTCTTGATGCCTGCTACAGTTTTTATGCTCTTTAGTGGCCAAGGAAGTTTGCTCCAGCCTTTTAT
ATATTATAGATTTCTTACTCTTCGATATTCCTCTAGAAGAAATCCATATTGTCGGAACTTGTTTAATGAA
CTGAGGATTGTGGTTGAACACATTATTATGAAGCCCTCCTGCCCACTGTTTGTGAGAAGACTATGTCTCC
AGAGCATCGCCTTTATCAGCAGACTGGCACCAACAGTTGCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203124 protein sequence
Red=Cloning site Green=Tags(s)

MADTTPNGPQGAGAVQFMMTNKLDTAMWLSRLFTVYCSALFVLPLLGLHEAASFYQRALLANALTSALRL
HQRLPHFQLSRAFLAQALLEDSCHYLLYSLIFVNSYPVTMSIFPVLLFSLLHAATYTKKVLDAKGSNSLP
LLRSFLDKLSTNQQNILKFIACNEIFLMPATVFMLFSGQGSLLQPFIYYRFLTLRYSSRRNPYCRNLFNE
LRIVVEHIIMKPSCPLFVRRLCLQSIAFISRLAPTVA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC005562
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC005562, AAH05562
RefSeq Size 1126 bp
RefSeq ORF 743 bp
Locus ID 67878
Cytogenetics 5 C3.1
MW 28 kDa
Summary Acts as a regulator of the tubular endoplasmic reticulum (ER) network. Suppresses the RTN3/4-induced formation of the ER tubules. Positively regulates PERK-mediated and IRE1-mediated unfolded protein response signaling.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC005562" in other vectors (4)
SKU Description Size Price
MC200767 Tmem33 (untagged) - Mouse transmembrane protein 33 (cDNA clone MGC:11715 IMAGE:3965864), (10ug) 10 ug
$300.00
MG203124 Tmem33 (tGFP-tagged) - Mouse transmembrane protein 33 (cDNA clone MGC:11715 IMAGE:3965864) 10 ug
$500.00
MR203124L3 Lenti ORF clone of Tmem33 (Myc-DDK-tagged) - Mouse transmembrane protein 33 (cDNA clone MGC:11715 IMAGE:3965864) 10 ug
$600.00
MR203124L4 Lenti ORF clone of Tmem33 (mGFP-tagged) - Mouse transmembrane protein 33 (cDNA clone MGC:11715 IMAGE:3965864) 10 ug
$600.00

Plasmid Map for MR203124

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203124
Circular map for MR203124

file-alt Citations

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