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Rnf182 (NM_183204) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203122
Rnf182 (Myc-DDK-tagged) - Mouse ring finger protein 182 (Rnf182)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rnf182
Synonyms C630023L15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203122 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAGCCAGCCGCTGGAAGAGCCTGCGGAGTCTCAGGCCTCGGATGAGCTCGAGTGTAAGATCTGTT
ACAATCGGTACAACCTGAAGCAGAGGAAGCCCAAGGTTCTGGAGTGTTGTCACAGGGTCTGCGCCAAATG
CCTCTACAAGATCATAGACTTTGGGGACTCTCCCCAGGGGGTCATCGTCTGTCCCTTCTGCAGGTTTGAG
ACCTGTCTGCCGGATGACGAAGTCAGCAGCCTGCCCGATGACAACAACATCCTTGTAAACTTGACCTGTG
GGGGCAAAGGGAAGAAATGCCTGCCTGAGAACCCCACTGAGCTGTTGCTCACTCCCAAGAGGCTGGCTTC
CCTTGTCAGCCCTTCCCACACGTCCTCTAATTGCTTGGTTATCACCATCATGGAGGTGCAGAGAGAGAGC
TCTCCATCACTCAGCTCCACCCCCGTGGTAGAATTCTACAGGCCAGCCAGTTTCGACTCTGTCACCACCG
TGTCCCATAACTGGACGGTGTGGAACTGCACCTCCTTGCTCTTTCAGACGTCCATCCGGGTGTTAGTGTG
GCTGCTAGGTTTGCTGTACTTCAGCTCCTTGCCCTTAGGGATCTACTTACTGGTGTCTAAGAAAGTCACC
CTGGGGGTGGTCTTCGTTAGCCTTGTCCCCTCTAGCCTTGTCATCCTGATGGTGTATGGTTTTTGCCAAT
GCGTTTGTCATGAATTTCTAGACTGTATGGCACTTCCTTCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203122 protein sequence
Red=Cloning site Green=Tags(s)

MASQPLEEPAESQASDELECKICYNRYNLKQRKPKVLECCHRVCAKCLYKIIDFGDSPQGVIVCPFCRFE
TCLPDDEVSSLPDDNNILVNLTCGGKGKKCLPENPTELLLTPKRLASLVSPSHTSSNCLVITIMEVQRES
SPSLSSTPVVEFYRPASFDSVTTVSHNWTVWNCTSLLFQTSIRVLVWLLGLLYFSSLPLGIYLLVSKKVT
LGVVFVSLVPSSLVILMVYGFCQCVCHEFLDCMALPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_183204
ORF Size 741 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_183204.4, NP_899027.1
RefSeq Size 3456 bp
RefSeq ORF 744 bp
Locus ID 328234
UniProt ID Q8C432
Cytogenetics 13 A4
MW 27.4 kDa
Summary E3 ubiquitin-protein ligase that mediates the ubiquitination of ATP6V0C and targets it to degradation via the ubiquitin-proteasome pathway.UniProtKB/Swiss-Prot Function
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"NM_183204" in other vectors (4)
SKU Description Size Price
MC214519 Rnf182 (untagged) - Mouse ring finger protein 182 (Rnf182), (10ug) 10 ug
$330.00
MG203122 Rnf182 (tGFP-tagged) - Mouse ring finger protein 182 (Rnf182) 10 ug
$500.00
MR203122L3 Lenti ORF clone of Rnf182 (Myc-DDK-tagged) - Mouse ring finger protein 182 (Rnf182) 10 ug
$600.00
MR203122L4 Lenti ORF clone of Rnf182 (mGFP-tagged) - Mouse ring finger protein 182 (Rnf182) 10 ug
$600.00

Plasmid Map for MR203122

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203122
Circular map for MR203122

file-alt Citations

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