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Mdfi (BC085233) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR203083
Mdfi (Myc-DDK-tagged) - Mouse MyoD family inhibitor (cDNA clone MGC:102509 IMAGE:5318215)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Mdfi or BC085233"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mdfi
Synonyms I-mf; I-mfa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR203083 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCAGGTGAGCGGTCAGTGCCCTTCTCGCTGCGACGCGCCTCATGGAGTCCCCAGCGCTGCCCTGG
ACCCAGCCCAGACCATGTCCCTCCTCCCTGGGCTGGAGGTAGCAAGATCCACTCACCCTGTAGAGGCATC
TTCTGAAGAGGGCTTCCCGGAGGAGGCGGCACCCTCCATGCCCCATGACAGTGGTCTCCGGGCTCAGCAG
GCTCTGAACAGCATTGACCTCGATGTCCCCACAGAAGCTGTGACGTGCCAGCCTCAAGGGAACCCCCAAG
GCTGCACCCCACTACTGCCAAATGGCTCCAGCCACGACCACCTCTCAGAACCGGGCAGTGCAGGGCATGC
GGGGAACGGTGCTCTGGGCGGGTCCAAGGCCCACCGGAAGTTGCAGACGCATCCATCTCTGGGCAGCCAG
GCTGGAAGGAAAAGCAGAGGCAGCGCCCGGTCAGCCTCACAGGTCCCTCTCCAGGCACAGGAAGATTGCT
GCGTCCACTGCATACTGTCCTGTCTATTCTGTGAGTTCCTGACGCTCTGTAACATCCTCCTGGACTGCGC
CACCTGTGGCTCCTGCAGCTCTGAGGACTCCTGCCTCTGCTGCTGCTGCTGTGGGTCCGGCGAGTGCGCG
GACTGTGACCTGCCCTGCGACCTGGACTGCGGCATCGTGGATGCCTGCTGCGAGTCCGCAGACTGCTTGG
AGATATGCATGGAGTGCTGTGGACTCTGTTTCTCCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR203083 protein sequence
Red=Cloning site Green=Tags(s)

MSQVSGQCPSRCDAPHGVPSAALDPAQTMSLLPGLEVARSTHPVEASSEEGFPEEAAPSMPHDSGLRAQQ
ALNSIDLDVPTEAVTCQPQGNPQGCTPLLPNGSSHDHLSEPGSAGHAGNGALGGSKAHRKLQTHPSLGSQ
AGRKSRGSARSASQVPLQAQEDCCVHCILSCLFCEFLTLCNILLDCATCGSCSSEDSCLCCCCCGSGECA
DCDLPCDLDCGIVDACCESADCLEICMECCGLCFSS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC085233
ORF Size 738 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC085233, AAH85233
RefSeq Size 1725 bp
RefSeq ORF 740 bp
Locus ID 17240
Cytogenetics 17 23.99 cM
MW 25.3 kDa
Summary Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways.UniProtKB/Swiss-Prot Function
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"BC085233" in other vectors (5)
SKU Description Size Price
MG203083 Mdfi (tGFP-tagged) - Mouse MyoD family inhibitor (cDNA clone MGC:102509 IMAGE:5318215) 10 ug
$500.00
MR203083L1 Lenti ORF clone of Mdfi (Myc-DDK-tagged) - Mouse MyoD family inhibitor (cDNA clone MGC:102509 IMAGE:5318215) 10 ug
$600.00
MR203083L2 Lenti ORF clone of Mdfi (mGFP-tagged) - Mouse MyoD family inhibitor (cDNA clone MGC:102509 IMAGE:5318215) 10 ug
$600.00
MR203083L3 Lenti ORF clone of Mdfi (Myc-DDK-tagged) - Mouse MyoD family inhibitor (cDNA clone MGC:102509 IMAGE:5318215) 10 ug
$600.00
MR203083L4 Lenti ORF clone of Mdfi (mGFP-tagged) - Mouse MyoD family inhibitor (cDNA clone MGC:102509 IMAGE:5318215) 10 ug
$600.00

Plasmid Map for MR203083

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR203083
Circular map for MR203083

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