Tspan4 (NM_053082) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR202919
Tspan4 (Myc-DDK-tagged) - Mouse tetraspanin 4 (Tspan4)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tspan4
Synonyms AI325509; AI746565; D130042I01Rik; NAG-2; Tm4sf7; Tspan-4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202919 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGCGGCTGCCTCCAGGGCGTCAAGTACCTCATGTTCGCCTTCAACCTGCTCTTCTGGCTGGGTG
GCTGTGGTGTCCTGGGTGTTGGCATCTGGTTGGCTGCCACACAGGGAAACTTTGCCACCTTATCATCCTC
ATTTCCATCCTTGTCGGCTGCCAACCTGCTCATCGTCACCGGGACCTTCGTCATGGCCATCGGCTTCGTG
GGCTGCATTGGGGCCCTCAAGGAGAACAAGTGCCTACTGCTCACTTTCTTTGTGCTGCTGCTGCTAGTGT
TCCTGCTGGAAGCCACCATTGCTGTGCTCTTCTTTGCCTACAGTGACAAGATTGACAGTTATGCCCAACA
AGACCTGAAGAAGGGCCTGCATCTGTATGGCACACAGGGCAACGTGGGCCTCACCAATGCCTGGAGCATC
ATCCAGACTGATTTCCGATGCTGTGGAGTTTCCAATTACACTGATTGGTTTGAGGTATACAATGCCACTC
GTGTGCCTGACTCCTGCTGTCTGGAGTTCAGTGATAGCTGTGGGTTACATGAACCTGGTACCTGGTGGAA
GTCGCCCTGTTATGAGACAGTGAAGGCCTGGCTCCAGGAGAACCTGCTAGCTGTGGGCATCTTTGGACTG
TGCACGGCACTGGTGCAGATTCTGGGCCTCACCTTCGCTATGACCATGTACTGCCAGGTGGTAAAGGCGG
ACACCTACTGTGCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR202919 protein sequence
Red=Cloning site Green=Tags(s)

MARGCLQGVKYLMFAFNLLFWLGGCGVLGVGIWLAATQGNFATLSSSFPSLSAANLLIVTGTFVMAIGFV
GCIGALKENKCLLLTFFVLLLLVFLLEATIAVLFFAYSDKIDSYAQQDLKKGLHLYGTQGNVGLTNAWSI
IQTDFRCCGVSNYTDWFEVYNATRVPDSCCLEFSDSCGLHEPGTWWKSPCYETVKAWLQENLLAVGIFGL
CTALVQILGLTFAMTMYCQVVKADTYCA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_053082
ORF Size 714 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_053082.3
RefSeq Size 1486 bp
RefSeq ORF 717 bp
Locus ID 64540
UniProt ID Q9DCK3
Cytogenetics 7 F5
MW 26.1 kDa
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Documents
Resources
"NM_053082" in other vectors (5)
SKU Description Size Price
MC200431 Tspan4 (untagged) - Mouse tetraspanin 4 (Tspan4), (10ug) 10 ug
$300.00
MG202919 Tspan4 (tGFP-tagged) - Mouse tetraspanin 4 (Tspan4) 10 ug
$489.00 $500.00
MR202919L3 Lenti ORF clone of Tspan4 (Myc-DDK-tagged) - Mouse tetraspanin 4 (Tspan4) 10 ug
$600.00
MR202919L4 Lenti ORF clone of Tspan4 (mGFP-tagged) - Mouse tetraspanin 4 (Tspan4) 10 ug
$600.00
MR202920 Tspan4 (Myc-DDK-tagged) - Mouse tetraspanin 4 (Tspan4) 10 ug
$330.00

Plasmid Map for MR202919

Download sequence for

Cloning scheme for MR202919

Circular map for MR202919

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