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Tk1 (BC091758) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202823
Tk1 (Myc-DDK-tagged) - Mouse thymidine kinase 1 (cDNA clone MGC:102559 IMAGE:4978985)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tk1
Synonyms Tk1a, Tk1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202823 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTACATCAATCTGCCCACCGTGCTGCCCAGCTCCCCCAGCAAGACTCGGGGGCAGATTCAGGTGA
TTCTCGGGCTCATGTTCTCAGGGAAAAGCACAGAGCTGATGAGAAGAGTCCGGCGCTTCCAGATCGCCCA
GTACAAGTGCCTGGTCATCAAGTATGCCAAAGACACGCGCTATAGCAACAGCTTCTCCACACATGATCGG
AACACCATGGACGCATTGCCAGCCTGCATGCTCCGCGATGTGACCCAGGAGTCCTTGGGTGTGGCCGTCA
TTGGCATCGATGAGGGGCAGTTTTTTCCTGACATTGTGGATTTCTGTGAAATGATGGCCAACGAGGGCAA
GACAGTAATTGTGGCAGCGCTGGATGGGACCTTCCAGAGGAAGGCTTTCGGCAGCATCTTGAACCTGGTG
CCCCTGGCGGAGAGTGTGGTGAAGCTCACCGCTGTGTGCATGGAGTGCTTCCGAGAAGCTGCCTACACGA
AGAGGCTGGGCCTGGAGAAAGAGGTGGAGGTGATTGGCGGAGCCGACAAGTATCACTCCGTGTGCCGCCT
GTGCTACTTTAAGAAGTCTTCAGCCCAGACTGCTGGCTCAGACAACAAGAACTGTCTGGTGCTGGGGCAG
CCGGGAGAGGCCTTGGTTGTCAGGAAGCTCTTTGCCTCTCAGCAAGTCCTACAATACAACTCTGCCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202823 protein sequence
Red=Cloning site Green=Tags(s)

MSYINLPTVLPSSPSKTRGQIQVILGLMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSNSFSTHDR
NTMDALPACMLRDVTQESLGVAVIGIDEGQFFPDIVDFCEMMANEGKTVIVAALDGTFQRKAFGSILNLV
PLAESVVKLTAVCMECFREAAYTKRLGLEKEVEVIGGADKYHSVCRLCYFKKSSAQTAGSDNKNCLVLGQ
PGEALVVRKLFASQQVLQYNSAN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC091758
ORF Size 699 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC091758, AAH91758
RefSeq Size 1209 bp
RefSeq ORF 701 bp
Locus ID 21877
Cytogenetics 11 82.96 cM
MW 25.8 kDa
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"BC091758" in other vectors (3)
SKU Description Size Price
MG202823 Tk1 (tGFP-tagged) - Mouse thymidine kinase 1 (cDNA clone MGC:102559 IMAGE:4978985) 10 ug
$500.00
MR202823L3 Lenti ORF clone of Tk1 (Myc-DDK-tagged) - Mouse thymidine kinase 1 (cDNA clone MGC:102559 IMAGE:4978985) 10 ug
$600.00
MR202823L4 Lenti ORF clone of Tk1 (mGFP-tagged) - Mouse thymidine kinase 1 (cDNA clone MGC:102559 IMAGE:4978985) 10 ug
$600.00

Plasmid Map for MR202823

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202823
Circular map for MR202823

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