Gap43 (NM_008083) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR202705
Gap43 (Myc-DDK-tagged) - Mouse growth associated protein 43 (Gap43)
  $450.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gap43
Synonyms B-50; Basp2; GAP-43
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202705 representing NM_008083
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGTGCTGTATGAGAAGAACCAAACAGGTTGAAAAGAATGATGAGGACCAAAAGATTGAACAAGATG
GTGTCAAGCCGGAAGATAAGGCTCATAAGGCTGCGACCAAAATTCAGGCTAGCTTCCGTGGACACATAAC
AAGGAAAAAGCTCAAAGGCGAGAAGAAGGGTGATGCACCAGCTGCTGAGGCCGAGGCCAAGGAGAAGGAT
GATGCTCCCGTTGCTGATGGTGTGGAGAAGAAGGAGGGAGATGGCTCTGCTACTACCGATGCAGCCCCAG
CCACCAGCCCCAAGGCTGAGGAGCCCAGCAAGGCAGGAGATGCACCTTCTGAGGAGAAGAAGGGTGAAGG
GGATGCGGCCCCCTCCGAGGAAAAGGCCGGCTCAGCGGAGACAGAAAGTGCTGCTAAAGCTACCACTGAT
AACTCCCCGTCCTCCAAGGCTGAAGATGGCCCAGCCAAGGAGGAGCCTAAACAAGCCGATGTGCCTGCTG
CTGTCACTGATGCTGCTGCCACCACCCCTGCTGCAGAGGATGCTGCCACCAAGGCAGCGCAGCCTCCAAC
GGAGACTGCAGAAAGCAGCCAAGCTGAGGAGGAGAAAGACGCTGTAGACGAAGCCAAACCTAAGGAAAGT
GCCCGACAGGATGAGGGTAAAGAAGACCCCGAGGCTGACCAAGAACATGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR202705 representing NM_008083
Red=Cloning site Green=Tags(s)

MLCCMRRTKQVEKNDEDQKIEQDGVKPEDKAHKAATKIQASFRGHITRKKLKGEKKGDAPAAEAEAKEKD
DAPVADGVEKKEGDGSATTDAAPATSPKAEEPSKAGDAPSEEKKGEGDAAPSEEKAGSAETESAAKATTD
NSPSSKAEDGPAKEEPKQADVPAAVTDAAATTPAAEDAATKAAQPPTETAESSQAEEEKDAVDEAKPKES
ARQDEGKEDPEADQEHA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008083
ORF Size 681 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008083.2, NP_032109.1
RefSeq Size 1316 bp
RefSeq ORF 684 bp
Locus ID 14432
UniProt ID P06837
Cytogenetics 16 28.37 cM
MW 24.1 kDa
Summary This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008083" in other vectors (4)
SKU Description Size Price
MC202783 Gap43 (untagged) - Mouse growth associated protein 43 (Gap43), (10ug) 10 ug
$450.00
MG202705 Gap43 (tGFP-tagged) - Mouse growth associated protein 43 (Gap43) 10 ug
$650.00
MR202705L3 Lenti ORF clone of Gap43 (Myc-DDK-tagged) - Mouse growth associated protein 43 (Gap43) 10 ug
$750.00
MR202705L4 Lenti ORF clone of Gap43 (mGFP-tagged) - Mouse growth associated protein 43 (Gap43) 10 ug
$750.00

Plasmid Map for MR202705

Download sequence for

Cloning scheme for MR202705

Circular map for MR202705

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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