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Vti1a (BC089321) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202611
Vti1a (Myc-DDK-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (cDNA clone MGC:102006 IMAGE:6839437)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
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Bulk Requests & Clone Modifications

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Other products for "Vti1a or BC089321"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vti1a
Synonyms 4921537J05Rik, Vti1, MVti1a, MVti1, Vti1-rp2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202611 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTCCGACTTCGAAGGGTACGAGCAGGACTTCGCAGTGCTCACTGCTGAGATCACCAGCAAGATTG
CAAGGGTCCCTCGGCTCCCTCCCGATGAGAAGAAGCAAATGGTTGCCAATGTGGAGAAACAGCTTGAAGA
GGCCAGAGAACTGCTTGAACAGATGGATTTGGAAGTTCGAGAAATTCCACCCCAAAGTCGAGGAATGTAT
AGCAACAGGATGAGAAGCTACAAGCAAGAAATGGGGAAACTGGAAACGGATTTTAAAAGGTCACGGATCG
CCTACAGTGACGAAGTACGGAATGAGCTCCTAGGGGATGCTGGGAATTCCTCCGAGAACCAGTTGATAAA
ATTACGTGAAGAGAGGGCACATCTGCTGGATAACACGGAGAGGCTGGAAAGGTCGTCTCGGAGACTAGAG
GCTGGGTACCAGATAGCAGTGGAGACTGAGCAAATCGGCCAAGAGATGTTGGAAAACCTGAGTCATGACA
GAGAAAAGATACAGCGGGCACGTGACCGACTCCGGGACGCAGATGCAAACCTGGGGAAGAGCTCTCGGAT
TCTGACGGGGATGCTGCGAAGAATCATCCAGAACCGTATCCTGCTCGTCATCTTGGGGATCATCGTGGTC
ATCGCCATCCTGACGGCCATCGCGTTTTTCGTCAAAGGACAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202611 protein sequence
Red=Cloning site Green=Tags(s)

MSSDFEGYEQDFAVLTAEITSKIARVPRLPPDEKKQMVANVEKQLEEARELLEQMDLEVREIPPQSRGMY
SNRMRSYKQEMGKLETDFKRSRIAYSDEVRNELLGDAGNSSENQLIKLREERAHLLDNTERLERSSRRLE
AGYQIAVETEQIGQEMLENLSHDREKIQRARDRLRDADANLGKSSRILTGMLRRIIQNRILLVILGIIVV
IAILTAIAFFVKGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC089321
ORF Size 672 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC089321, AAH89321
RefSeq Size 4196 bp
RefSeq ORF 674 bp
Locus ID 53611
Cytogenetics 19 D2
MW 25.9 kDa
Summary V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence.UniProtKB/Swiss-Prot Function
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"BC089321" in other vectors (3)
SKU Description Size Price
MG202611 Vti1a (tGFP-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (cDNA clone MGC:102006 IMAGE:6839437) 10 ug
$500.00
MR202611L3 Lenti ORF clone of Vti1a (Myc-DDK-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (cDNA clone MGC:102006 IMAGE:6839437) 10 ug
$600.00
MR202611L4 Lenti ORF clone of Vti1a (mGFP-tagged) - Mouse vesicle transport through interaction with t-SNAREs homolog 1A (yeast) (cDNA clone MGC:102006 IMAGE:6839437) 10 ug
$600.00

Plasmid Map for MR202611

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202611
Circular map for MR202611

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