Cidea (NM_007702) Mouse Tagged ORF Clone
- TrueORF®
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cidea |
| Synonyms | AW212747 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_007702 |
| ORF Size | 651 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_007702.2 |
| RefSeq Size | 1114 bp |
| RefSeq ORF | 654 bp |
| Locus ID | 12683 |
| UniProt ID | O70302 |
| Cytogenetics | 18 E1 |
| MW | 25.1 kDa |
| Summary | Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_007702" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC208005 | Cidea (untagged) - Mouse cell death-inducing DNA fragmentation factor, alpha subunit-like effector A (Cidea), (10ug) | 10 ug |
$450.00
|
|
| MG202435 | Cidea (tGFP-tagged) - Mouse cell death-inducing DNA fragmentation factor, alpha subunit-like effector A (Cidea) | 10 ug |
$650.00
|
|
| MR202435L3 | Lenti ORF clone of Cidea (Myc-DDK-tagged) - Mouse cell death-inducing DNA fragmentation factor, alpha subunit-like effector A (Cidea) | 10 ug |
$750.00
|
|
| MR202435L4 | Lenti ORF clone of Cidea (mGFP-tagged) - Mouse cell death-inducing DNA fragmentation factor, alpha subunit-like effector A (Cidea) | 10 ug |
$750.00
|
|
Citations
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