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Grpel1 (NM_024478) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202418
Grpel1 (Myc-DDK-tagged) - Mouse GrpE-like 1, mitochondrial (Grpel1), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Grpel1 or NM_024478"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Grpel1
Synonyms AA408748; mt-GrpE#1; mt-Grpel1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202418 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTCGGTGTGTGAGGCTGGCGCGTCGCAGCCTCCCGGCTTTGGCACTGTCGTTCAGGCCTTCTC
CTCGCTTGTTGTGCACAGCTACGAAACAGAAGAACAATGGCCAGAACCTGGACGAGGACTTGGGTCATTG
TGAGCCAAAGACAGATCCACCCTCTGCAGACAAGACCCTCCTGGAAGAGAAGGCAAAGCTGGAAGAGCAG
CTGAGGGAGACCATGGAAAAATACAAACGTGCTTTGGCAGATACTGAGAATCTACGGCAGAGAAGCCAGA
AGCTGGTAGAGGAGGCCAAGTTATATGGCATCCAGGGTTTCTGCAAGGACTTGCTGGAGGTTGCAGACAT
CCTGGAGAAGGCAACCCAGAGTGTTCCAAAGGAGGAGATCAGCAACAACAACCCTCACCTGAAGAGTCTT
TATGAAGGGCTCGTGATGACTGAAGTCCAGATTCAGAAGGTGTTCACAAAACACGGCTTGCTCAGGCTTG
ACCCAATTGGGGCCAAGTTCGACCCTTACGAACATGAGGCCTTGTTCCACACCCCTGTGGAGGGGAAAGA
ACCAGGCACTGTGGCACTAGTTAGTAAGGTGGGCTACAAGCTGCACGGACGCACCCTGAGGCCAGCTTTG
GTGGGGGTGGTGAAGGACGCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202418 protein sequence
Red=Cloning site Green=Tags(s)

MAARCVRLARRSLPALALSFRPSPRLLCTATKQKNNGQNLDEDLGHCEPKTDPPSADKTLLEEKAKLEEQ
LRETMEKYKRALADTENLRQRSQKLVEEAKLYGIQGFCKDLLEVADILEKATQSVPKEEISNNNPHLKSL
YEGLVMTEVQIQKVFTKHGLLRLDPIGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYKLHGRTLRPAL
VGVVKDA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024478
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024478.1
RefSeq Size 3338 bp
RefSeq ORF 654 bp
Locus ID 17713
UniProt ID Q99LP6
Cytogenetics 5 B3
MW 24.3 kDa
Summary Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins (By similarity).UniProtKB/Swiss-Prot Function
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"NM_024478" in other vectors (4)
SKU Description Size Price
MC200232 Grpel1 (untagged) - Mouse GrpE-like 1, mitochondrial (Grpel1), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG202418 Grpel1 (tGFP-tagged) - Mouse GrpE-like 1, mitochondrial (Grpel1) 10 ug
$500.00
MR202418L3 Lenti ORF clone of Grpel1 (Myc-DDK-tagged) - Mouse GrpE-like 1, mitochondrial (Grpel1), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR202418L4 Lenti ORF clone of Grpel1 (mGFP-tagged) - Mouse GrpE-like 1, mitochondrial (Grpel1), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR202418

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202418
Circular map for MR202418

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