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Nola1 (BC021873) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202402
Nola1 (Myc-DDK-tagged) - Mouse nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (cDNA clone MGC:28064 IMAGE:3709271)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nola1
Synonyms GAR1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202402 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTTTCCGAGGCGGAGGTCGCGGAGGCTTTAATCGCGGTGGTGGAGGCGGAGGCTTCAACCGCGGAG
GCGGCGGCGGCAGTTTCAGGGGCGGAGGCGGCGGCGGCAGTTTCAGGGGCGGCGGCCGAGGAGGATTTGG
ACGAGGGGGCGGTCGTGGAGGCTTTAATAAATTTCAAGATCAAGGGCCTCCAGAACGTGTCGTCTTGTTA
GGAGAATTCATGCATCCCTGTGAAGATGACATCGTGTGTAAATGTACCACCGAGGAGAACAAGGTGCCCT
ACTTCAACGCCCCTGTTTACTTAGAAAACAAAGAGCAAGTCGGGAAAGTGGATGAGATATTTGGACAGCT
TAGAGATTTTTATTTTTCAGTTAAGTTGTCAGAAAACATGAAGGCATCTTCCTTTAAAAAGCTACAGAAG
TTCTATATAGACCCATACAAGCTGCTGCCGCTGCAGAGGTTTCTGCCTCGTCCTCCTGGTGAGAAAGGAC
CTCCCAGAGGTGGCGGCGGTGGCGGCAGGGGAGGTCGAGGAGGAGGAAGAGGAGGCGGTGGCCGAGGTGG
TGGAAGAGGTGGTGGTTTTAGAGGAGGCAGAGGAGGAGGTGGGGGCTTCAGAGGAGGCCGAGGAGGCGGC
GGATTCCGAGGAAGGGGACAT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202402 protein sequence
Red=Cloning site Green=Tags(s)

MSFRGGGRGGFNRGGGGGGFNRGGGGGSFRGGGGGGSFRGGGRGGFGRGGGRGGFNKFQDQGPPERVVLL
GEFMHPCEDDIVCKCTTEENKVPYFNAPVYLENKEQVGKVDEIFGQLRDFYFSVKLSENMKASSFKKLQK
FYIDPYKLLPLQRFLPRPPGEKGPPRGGGGGGRGGRGGGRGGGGRGGGRGGGFRGGRGGGGGFRGGRGGG
GFRGRGH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC021873
ORF Size 651 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC021873, AAH21873
RefSeq Size 1145 bp
RefSeq ORF 653 bp
Locus ID 68147
Cytogenetics 3 G3
MW 22.3 kDa
Summary Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme (By similarity).UniProtKB/Swiss-Prot Function
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"BC021873" in other vectors (4)
SKU Description Size Price
MC206920 Nola1 (untagged) - Mouse nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (cDNA clone MGC:28064 IMAGE:3709271),, (10ug) 10 ug
$330.00
MG202402 Nola1 (tGFP-tagged) - Mouse nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (cDNA clone MGC:28064 IMAGE:3709271) 10 ug
$500.00
MR202402L3 Lenti ORF clone of Nola1 (Myc-DDK-tagged) - Mouse nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (cDNA clone MGC:28064 IMAGE:3709271) 10 ug
$600.00
MR202402L4 Lenti ORF clone of Nola1 (mGFP-tagged) - Mouse nucleolar protein family A, member 1 (H/ACA small nucleolar RNPs) (cDNA clone MGC:28064 IMAGE:3709271) 10 ug
$600.00

Plasmid Map for MR202402

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202402
Circular map for MR202402

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