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Efcab9 (NM_027031) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202382
Efcab9 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 9 (Efcab9)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Efcab9
Synonyms 1700007I06Rik; 4930403C08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202382 representing NM_027031
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACTGACTCCGGGGTGTTTTCTGTGGTACCTTTACATGGACAAAATCTACTGCTTACTGTCTTTGA
GAAACGTGAAAGCCTTGATGGTGTACTTTCATCTTCTGGATGTGCACCACAGGAACACCTTGAATGATGT
GCTCTTCTTTCATTTCCTCCAGCACGTGACTAACCTGAACAAGTCACAGATCGGGATGATATTTGACCTC
CTGGACTGGACAGCTGTAGGAGAGATTGGTTTTGACCAGTTCTATGTGTTGATTTGCATACTGCTGGCAC
ATCAGGACCATCTGGAAGACCACTTTATGTACCGCCATTCCCGGCCAGTCTTTGAGCTGCTTGATCTGGA
CGGGGAGATGAATATAGGTGCGGCCAATTTCCAAAACTACAGATTTCTCTTCAATATTAAAAAGCAGGAA
CTCCGAGACCTTTTCCATGACTTTGACATCACAGGTGACCGACTGCTTAATTACAAGGAATTTAAGCTGT
ACACAATCTTCTGCACCGACAAATCCATAGACAGAAAGAAAAGGAGGAAAGACAGAGAGGCAGCGAGAGA
GAGAGAAAAAGAGAAAGGGAAAGATAAAGAGAAGTATCTTCATCTCAAGAAGATATATTCATCTATGTTA
AGCCACAGGAGTATCCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202382 representing NM_027031
Red=Cloning site Green=Tags(s)

MKLTPGCFLWYLYMDKIYCLLSLRNVKALMVYFHLLDVHHRNTLNDVLFFHFLQHVTNLNKSQIGMIFDL
LDWTAVGEIGFDQFYVLICILLAHQDHLEDHFMYRHSRPVFELLDLDGEMNIGAANFQNYRFLFNIKKQE
LRDLFHDFDITGDRLLNYKEFKLYTIFCTDKSIDRKKRRKDREAAREREKEKGKDKEKYLHLKKIYSSML
SHRSIL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027031
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027031.3, NP_081307.2
RefSeq Size 767 bp
RefSeq ORF 651 bp
Locus ID 69306
UniProt ID Q9DAM2
Cytogenetics 11 A4
MW 26.1 kDa
Summary pH-dependent Ca(2+) sensor required to activate the CatSper channel, a complex involved in sperm cell hyperactivation (PubMed:31056283). Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization (PubMed:31056283). Associates with the CatSper complex via direct interaction with CATSPERZ, and senses intracellular Ca(2+) (PubMed:31056283). Together with CATSPERZ, associates with the CatSper channel pore and is required for the two-row structure of each single CatSper channel (PubMed:31056283).UniProtKB/Swiss-Prot Function
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Documents
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"NM_027031" in other vectors (4)
SKU Description Size Price
MC210889 Efcab9 (untagged) - Mouse EF-hand calcium binding domain 9 (Efcab9), (10ug) 10 ug
$330.00
MG202382 Efcab9 (tGFP-tagged) - Mouse RIKEN cDNA 1700007I06 gene (1700007I06Rik) 10 ug
$500.00
MR202382L3 Lenti ORF clone of Efcab9 (Myc-DDK-tagged) - Mouse EF-hand calcium binding domain 9 (Efcab9) 10 ug
$600.00
MR202382L4 Lenti ORF clone of Efcab9 (mGFP-tagged) - Mouse EF-hand calcium binding domain 9 (Efcab9) 10 ug
$600.00

Plasmid Map for MR202382

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202382
Circular map for MR202382

file-alt Citations

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