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Rab25 (NM_016899) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202325
Rab25 (Myc-DDK-tagged) - Mouse RAB25, member RAS oncogene family (Rab25)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Rab25 or NM_016899"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab25
Synonyms AV012978
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202325 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAATCGAACAGATGAAGATTATAACTTTGTCTTTAAGGTGGTGCTGATCGGCGAGTCAGGCGTGG
GCAAGACCAATCTGCTGTCCCGGTTCACCCGCAATGAGTTCAGCCACGACAGTCGCACCACCATCGGGGT
TGAGTTCTCCACCCGCACTGTAATGCTGGGCACCGCTGCTGTCAAGGCACAGATCTGGGACACGGCCGGC
CTGGAGAGATACAGAGCCATCACCTCTGCGTACTATCGTGGGGCCGTGGGGGCACTCCTGGTATTTGACC
TGACCAAGCACCAGACCTACGCTGTGGTGGAGCGCTGGCTAAAGGAGCTGTATGACCATGCCGAAGCCAC
GATTGTTGTCATGCTCGTGGGGAACAAAAGTGACCTCAGCCAGGCCCGAGAGGTCCCTACTGAGGAGGCC
TGCATGTTTGCTGAAAACAATGGCCTGCTGTTCCTGGAGACGTCAGCCCTCGACTCCACCAATGTTGAGC
TAGCCTTCCAGACTGTCCTCAAAGAGATCTTTGCAAAGGTGTCCAAGCAGAAGCAGAACAGCACCCGGAC
CAGTGCCATCACCCTGGGCAATGCCCAAGCTGGACAGGATCCTGGCCCTGGGGAAAAGAGGGCCTGTTGC
ATCAGCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202325 protein sequence
Red=Cloning site Green=Tags(s)

MGNRTDEDYNFVFKVVLIGESGVGKTNLLSRFTRNEFSHDSRTTIGVEFSTRTVMLGTAAVKAQIWDTAG
LERYRAITSAYYRGAVGALLVFDLTKHQTYAVVERWLKELYDHAEATIVVMLVGNKSDLSQAREVPTEEA
CMFAENNGLLFLETSALDSTNVELAFQTVLKEIFAKVSKQKQNSTRTSAITLGNAQAGQDPGPGEKRACC
ISL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016899
ORF Size 639 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016899.4
RefSeq Size 1060 bp
RefSeq ORF 642 bp
Locus ID 53868
UniProt ID Q9WTL2
Cytogenetics 3 F1
MW 23.5 kDa
Summary Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions (PubMed:22696678). May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis (By similarity).UniProtKB/Swiss-Prot Function
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"NM_016899" in other vectors (4)
SKU Description Size Price
MC200413 Rab25 (untagged) - Mouse RAB25, member RAS oncogene family (Rab25), (10ug) 10 ug
$300.00
MG202325 Rab25 (tGFP-tagged) - Mouse RAB25, member RAS oncogene family (Rab25) 10 ug
$500.00
MR202325L3 Lenti ORF clone of Rab25 (Myc-DDK-tagged) - Mouse RAB25, member RAS oncogene family (Rab25) 10 ug
$600.00
MR202325L4 Lenti ORF clone of Rab25 (mGFP-tagged) - Mouse RAB25, member RAS oncogene family (Rab25) 10 ug
$600.00

Plasmid Map for MR202325

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202325
Circular map for MR202325

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