Bag2 (NM_145392) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR202268
Bag2 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 2 (Bag2)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Bag2
Synonyms 2610042A13Rik; BC016230
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202268 representing NM_145392
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCAGGCGAAGATCAGCGCCAAGGCCCACGAGGGCCGCTTCTGCCGCTCGTCTTCCATGGCCGACC
GCTCCAGCCGCCTGCTGGAGAGTCTGGACCAGCTGGAGCTCAGGGTGGAAGCTTTGAGAGACGCAGCTAC
TGCTGTTGAGCAGGAGAAAGAAATCCTTCTGGAGATGATCCACAGTATCCAAAACAGCCAGGACATGAGG
CAGATTAGCGATGGAGAAAGAGAGGAACTAAACCTGACTGCCAACCGTCTGATGGGCCGGACCCTCACGG
TTGAGGTCTCGGTGGAAACGATCCGAAACCCCCAGCAGGAGGAATCCTTGAAGCATGCCACGAGGATTAT
AGACGAGGTGGTCAGCAAGTTCCTAGATGACCTGGGGAATGCCAAGAGCCACTTAATGTCACTTTACAGT
GCCTGCTCATCGGAGGTGCCGCCTGGGCCGGTGGACCAGAAGTTTCAATCGATAGTCATCGGTTGCGCTC
TTGAGGATCAGAAGAAAATCAAGAGGCGATTGGAGACTCTGCTGAGGAACATTGACAACTCCGACAAGGC
CATTAAACTCCTAGAGCATGCTAAAGGAGCTGGTTCCAAAAGCCTGCAGAACACTGACGGCAAATTTAAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR202268 representing NM_145392
Red=Cloning site Green=Tags(s)

MAQAKISAKAHEGRFCRSSSMADRSSRLLESLDQLELRVEALRDAATAVEQEKEILLEMIHSIQNSQDMR
QISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQEESLKHATRIIDEVVSKFLDDLGNAKSHLMSLYS
ACSSEVPPGPVDQKFQSIVIGCALEDQKKIKRRLETLLRNIDNSDKAIKLLEHAKGAGSKSLQNTDGKFN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145392
ORF Size 630 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145392.2, NP_663367.1
RefSeq Size 1815 bp
RefSeq ORF 633 bp
Locus ID 213539
UniProt ID Q91YN9
Cytogenetics 1 B
MW 23.9 kDa
Summary Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_145392" in other vectors (4)
SKU Description Size Price
MC208095 Bag2 (untagged) - Mouse BCL2-associated athanogene 2 (Bag2), (10ug) 10 ug
$330.00
MG202268 Bag2 (tGFP-tagged) - Mouse Bcl2-associated athanogene 2 (Bag2) 10 ug
$500.00
MR202268L3 Lenti ORF clone of Bag2 (Myc-DDK-tagged) - Mouse BCL2-associated athanogene 2 (Bag2) 10 ug
$600.00
MR202268L4 Lenti ORF clone of Bag2 (mGFP-tagged) - Mouse BCL2-associated athanogene 2 (Bag2) 10 ug
$600.00

Plasmid Map for MR202268

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Cloning scheme for MR202268

Circular map for MR202268

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