Rab6b (NM_173781) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR202222
Rab6b (Myc-DDK-tagged) - Mouse RAB6B, member RAS oncogene family (Rab6b)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rab6b
Synonyms 9630015C03; AI844641; C330006L04Rik; D9Bwg0185e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202222 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGCAGGGGGAGATTTTGGGAACCCGCTGCGAAAATTCAAGTTGGTTTTCCTAGGGGAGCAGAGCG
TCGGGAAGACGTCTCTGATCACGAGGTTCATGTATGACAGCTTCGACAACACCTACCAGGCAACCATCGG
GATTGACTTCTTGTCAAAAACCATGTACTTGGAAGACCGTACGGTTCGACTGCAGCTCTGGGACACTGCT
GGGCAAGAGAGGTTCCGCAGCCTGATCCCCAGCTACATCCGAGACTCCACGGTGGCTGTGGTGGTATATG
ACATTACTAATCTCAACTCCTTCCAGCAGACTTCTAAATGGATTGATGACGTCAGGACGGAGAGGGGCAG
TGACGTGATCATCATGCTTGTGGGTAACAAGACGGATCTGGCTGATAAGAGGCAGATAACCATCGAGGAA
GGAGAACAGCGGGCCAAAGAACTGAGCGTCATGTTCATCGAGACCAGCGCGAAGACTGGCTACAACGTGA
AGCAGCTCTTCCGACGTGTGGCGTCAGCTCTGCCCGGCATGGAGAATGTCCAGGAGAAGAGCAAAGAAGG
GATGATTGACATCAAGCTGGACAAGCCTCAGGAGCCCCCGGCCAGCGAGGGCGGCTGCTCCTGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR202222 protein sequence
Red=Cloning site Green=Tags(s)

MSAGGDFGNPLRKFKLVFLGEQSVGKTSLITRFMYDSFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTA
GQERFRSLIPSYIRDSTVAVVVYDITNLNSFQQTSKWIDDVRTERGSDVIIMLVGNKTDLADKRQITIEE
GEQRAKELSVMFIETSAKTGYNVKQLFRRVASALPGMENVQEKSKEGMIDIKLDKPQEPPASEGGCSC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173781
ORF Size 624 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173781.4, NP_776142.1
RefSeq Size 4734 bp
RefSeq ORF 627 bp
Locus ID 270192
UniProt ID P61294
Cytogenetics 9 54.88 cM
MW 23.5 kDa
Summary Seems to have a role in retrograde membrane traffic at the level of the Golgi complex. May function in retrograde transport in neuronal cells (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173781" in other vectors (4)
SKU Description Size Price
MC207946 Rab6b (untagged) - Mouse RAB6B, member RAS oncogene family (Rab6b), (10ug) 10 ug
$330.00
MG202222 Rab6b (tGFP-tagged) - Mouse RAB6B, member RAS oncogene family (Rab6b) 10 ug
$500.00
MR202222L3 Lenti ORF clone of Rab6b (Myc-DDK-tagged) - Mouse RAB6B, member RAS oncogene family (Rab6b) 10 ug
$600.00
MR202222L4 Lenti ORF clone of Rab6b (mGFP-tagged) - Mouse RAB6B, member RAS oncogene family (Rab6b) 10 ug
$600.00

Plasmid Map for MR202222

Download sequence for

Cloning scheme for MR202222

Circular map for MR202222

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