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D1Ertd622e (NM_133825) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR202194
D1Ertd622e (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, ERATO Doi 622, expressed (D1Ertd622e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "D1Ertd622e or NM_133825"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D1Ertd622e
Synonyms AI987691; AW047450
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202194 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGTAGATATAAATGGGGATTCCAGAAGTACCCTGACCACCCTGCCCTTGCCTGTGGCTGAGGGAA
GCTCTCCAGGAAAAGCAGAGGCAGAGAAACCCCGCTGCTCTAGCACACCCTGCTCGCCCATGCGCCGGAC
TGTGTCTGGTTATCAGATCCTCCACATGGACTCTAACTACTTGGTTGGCTTCACCACTGGTGAGGAACTC
CTGAAGTTAGCCCAGAAGTGCACAGGAGGGGAAGACAGCAAGGGAGAAGCCATGCCCGCCTTGCGCGCTA
AACAGCTGGACACGGGACTCGCGCGTTCCTCTCGTCTGTATAAAACTAGAAGTAGGTACTATCAGCCATA
TGAGATCCCAGCGGTTAATGGCAGGAGGCGAAGGCGGATGCCCAGCTCAGGAGACAAGTGCACCAAACCC
TTACCTTATGAACCTTATAAGGCCCTCCATGGGCCCTTGCCTCTATGTCTTCTTAAAGGTAAGAGGGCTC
ACTCCAAATCTCTGGACTACCTCAATCTAGATAAAATGAACATCAAGGAGCCAGCTGACACGGAAGTGCT
ACAGTACCAGCTTCAGCACCTGACCCTCCGAGGGGACCGAGTGTTTGCTAGAAACAATACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202194 protein sequence
Red=Cloning site Green=Tags(s)

MEVDINGDSRSTLTTLPLPVAEGSSPGKAEAEKPRCSSTPCSPMRRTVSGYQILHMDSNYLVGFTTGEEL
LKLAQKCTGGEDSKGEAMPALRAKQLDTGLARSSRLYKTRSRYYQPYEIPAVNGRRRRRMPSSGDKCTKP
LPYEPYKALHGPLPLCLLKGKRAHSKSLDYLNLDKMNIKEPADTEVLQYQLQHLTLRGDRVFARNNT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_133825
ORF Size 621 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_133825.3
RefSeq Size 2738 bp
RefSeq ORF 624 bp
Locus ID 52392
UniProt ID Q8VEB3
Cytogenetics 1 47.76 cM
MW 23.1 kDa
Summary Probably plays a role in trafficking of proteins via its interaction with UNC119 and UNC119B cargo adapters: may help the release of UNC119 and UNC119B cargo or the recycling of UNC119 and UNC119B. May play a role in ciliary membrane localization via its interaction with UNC119B and protein transport into photoreceptor cells (By similarity).UniProtKB/Swiss-Prot Function
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"NM_133825" in other vectors (4)
SKU Description Size Price
MC204244 D1Ertd622e (untagged) - Mouse DNA segment, Chr 1, ERATO Doi 622, expressed (D1Ertd622e), (10ug) 10 ug
$300.00
MG202194 D1Ertd622e (tGFP-tagged) - Mouse DNA segment, Chr 1, ERATO Doi 622, expressed (D1Ertd622e) 10 ug
$500.00
MR202194L3 Lenti ORF clone of D1Ertd622e (Myc-DDK-tagged) - Mouse DNA segment, Chr 1, ERATO Doi 622, expressed (D1Ertd622e) 10 ug
$600.00
MR202194L4 Lenti ORF clone of D1Ertd622e (mGFP-tagged) - Mouse DNA segment, Chr 1, ERATO Doi 622, expressed (D1Ertd622e) 10 ug
$600.00

Plasmid Map for MR202194

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202194
Circular map for MR202194

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