OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Clec2e (NM_153506) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR202134
Clec2e (Myc-DDK-tagged) - Mouse C-type lectin domain family 2, member e (Clec2e)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA363528 CLEC2E Antibody - middle region
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Clec2e or NM_153506"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clec2e
Synonyms clr-a; Clra
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR202134 representing NM_153506
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGTGCTGCAAAGGTTGAAGAGGCTTCCATGGGCATGCTGAAGACAGACCTCACTGCCCCAGACTGCC
TGCAGGAGGGAGAAATGGGTAAAAAACTCCAAGCAAAATGTCTCGGAATCATCTCCGCTGCATCTTGTGT
TAAACTTTACTGCTGCTATGGAGTGATCATGGTCCTTACTGTAGCTGTAGTTGCTCTTTCTGTTACTTTG
TCAGTGAGAAAGAAAAAACCAGTCATGGAGAGCTGTGAACCTTGCTATGCTGTATGCTCAAGTGGCTGGA
TTGGATTTGGAAATAAATGCTTTTATTTTTCTGAAGACATGGGAAACTGGACATTCAGTCAGTCCTCCTG
CATAGCACTAGATGCCCATCTAGCTCTATTTGACAGCTTGGAGGAACTGAATTTTCTGAAGAGATACAAG
GGGGCTTCTGACCACTGGATTGGACTACACAGAGAGTCTTCAGAACACCCTTGGATATGGACAGACAACA
CTGAGTATAACAACTTGGTTCTCACCCGAGGAGGAGGAGAATGTGCCTACTTGAGTAACAGGGGGATCTA
CAACAGCAGTGGGGATATACATAAAAAATGGATTTGTAACAAGCCCAACAACTACACCTTACAGCGCCCA
CTAATTGTAAATCCCGGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR202134 representing NM_153506
Red=Cloning site Green=Tags(s)

MSAAKVEEASMGMLKTDLTAPDCLQEGEMGKKLQAKCLGIISAASCVKLYCCYGVIMVLTVAVVALSVTL
SVRKKKPVMESCEPCYAVCSSGWIGFGNKCFYFSEDMGNWTFSQSSCIALDAHLALFDSLEELNFLKRYK
GASDHWIGLHRESSEHPWIWTDNTEYNNLVLTRGGGECAYLSNRGIYNSSGDIHKKWICNKPNNYTLQRP
LIVNPG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153506
ORF Size 648 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153506.4, NP_705726.3
RefSeq Size 2271 bp
RefSeq ORF 651 bp
Locus ID 232409
Cytogenetics 6 F3
MW 24.4 kDa
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_153506" in other vectors (4)
SKU Description Size Price
MC205551 Clec2e (untagged) - Mouse C-type lectin domain family 2, member e (Clec2e), (10ug) 10 ug
$300.00
MG202134 Clec2e (tGFP-tagged) - Mouse C-type lectin domain family 2, member e (Clec2e) 10 ug
$500.00
MR202134L3 Lenti ORF clone of Clec2e (Myc-DDK-tagged) - Mouse C-type lectin domain family 2, member e (Clec2e) 10 ug
$600.00
MR202134L4 Lenti ORF clone of Clec2e (mGFP-tagged) - Mouse C-type lectin domain family 2, member e (Clec2e) 10 ug
$600.00

Plasmid Map for MR202134

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR202134
Circular map for MR202134

file-alt Citations

Microbiota Inhibit Epithelial Pathogen Adherence by Epigenetically Regulating C-Type Lectin Expression
Woo, Eshleman, Rice et al
Front Immunol (2019) 10, 928 DOI: 10.3389/fimmu.2019.00928
AI Summary: OriGene’s anti-DDK (FLAG) antibody was used in western blot analyses to detect tagged proteins, contributing to the study’s investigation of protein expression related to pathogen adherence in intestinal epithelial cells.
Snippet: For western blot analyses, total cell lysates were probed with anti-histone H3 (Santa Cruz) and anti-DDK (FLAG) (Origene) and imaged using an Odyssey Fc imager (LICOR)
FigureFigureFigure
WB
Showing 1-1 of 1 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products