Cdkn1b (NM_009875) Mouse Tagged ORF Clone
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cdkn1b |
| Synonyms | AA408329; AI843786; Kip1; p27; p27Kip1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009875 |
| ORF Size | 591 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009875.1 |
| RefSeq Size | 2405 bp |
| RefSeq ORF | 594 bp |
| Locus ID | 12576 |
| UniProt ID | P46414 |
| Cytogenetics | 6 65.77 cM |
| MW | 22.2 kDa |
| Summary | Important regulator of cell cycle progression (PubMed:8033213, PubMed:12972555). Inhibits the kinase activity of CDK2 bound to cyclin A, but has little inhibitory activity on CDK2 bound to SPDYA (By similarity). Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes (PubMed:8033213). Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009875" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC204260 | Cdkn1b (untagged) - Mouse cyclin-dependent kinase inhibitor 1B (Cdkn1b), (10ug) | 10 ug |
$450.00
|
|
| MG201957 | Cdkn1b (tGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 1B (Cdkn1b) | 10 ug |
$650.00
|
|
| MR201957L3 | Lenti ORF clone of Cdkn1b (Myc-DDK-tagged) - Mouse cyclin-dependent kinase inhibitor 1B (Cdkn1b) | 10 ug |
$750.00
|
|
| MR201957L4 | Lenti ORF clone of Cdkn1b (mGFP-tagged) - Mouse cyclin-dependent kinase inhibitor 1B (Cdkn1b) | 10 ug |
$750.00
|
|
Citations
p21 produces a bioactive secretome that places stressed cells under immunosurveillance
Sturmlechner, Zhang, Sine et al
Science (2021) 374 (6567), eabb3420 DOI: 10.1126/science.abb3420
Sturmlechner, Zhang, Sine et al
Science (2021) 374 (6567), eabb3420 DOI: 10.1126/science.abb3420
Cited by 291
AI Summary: OriGene’s Myc-Flag-tagged cDNA for mouse p21 (#MR227529) was used in this study to generate constructs for investigating the role of p21 in producing a bioactive secretome involved in immunosurveillance of stressed cells.
Snippet: The Myc-Flag-tagged cDNA for mouse p21 was obtained from Origene (#MR227529) and subcloned into the lentiviral pTSIN-PGK-puro2 backbone (43, 67) or dox-inducible pTRIPZ-PKG-puro backbone (modified from GE Dharmacon) (68). Similarly, the Myc-Flag- Save;- The Myc-Flag-tagged cDNA for mouse p21 was obtained from Origene (#MR227529) and subcloned into the lentiviral pTSIN-PGK-puro2 backbone (43, 67) or dox-inducible pTRIPZ-PKG-puro backbone (modified from GE Dharmacon) (68). …
Mechanics of a multilayer epithelium instruct tumour architecture and function
Fiore, Krajnc, Quiroz et al
Nature (2020) 585 (7825), 433-439 DOI: 10.1038/s41586-020-2695-9
Fiore, Krajnc, Quiroz et al
Nature (2020) 585 (7825), 433-439 DOI: 10.1038/s41586-020-2695-9
Cited by 167
AI Summary: In this study, OriGene products such as the shRNA plasmids (TRCN0000059008 and TRCN0000059009) were used to genetically manipulate basement membrane-associated genes, enabling the authors to investigate how changes in basement membrane stiffness influence tumour architecture and progression.
Snippet: Loss of normal tissue architecture is a hallmark of oncogenic transformation1. In developing organisms, tissues architectures are sculpted by mechanical forces during morphogenesis2. However, the origins and consequences of tissue architecture during tumorigenesis remain elusive. In skin, premalignant basal cell carcinomas form 'buds', while invasive squamous cell carcinomas initiate as 'folds'. H...
Showing 1-2 of 2 papers.
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