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Spint2 (NM_001082548) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201901
Spint2 (Myc-DDK-tagged) - Mouse serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Other products for "Spint2 or NM_001082548"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Spint2
Synonyms AL024025; C76321; HAI-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201901 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCAGCTTTGTGAGCTGCGGCGGGGCCGGGCGCTCCTCGCCTTGGTAGCTTCGCTGCTCCTCTCCG
GGGCCCAGGTGGCCAGCCGAGAACTAGACGTCCACGAGAACACCACTGATGACATGGCCAGGAACAGGAA
TGGAGCGGACTCTTCTGTCCTGAGTGTTCCCAGAAAGCAGAGTGCTGAGGACCTGTCTGCTGAGATCTTC
AACTATGAAGAATACTGTGTCCCGAAGGCCGTCACTGGGCCGTGCCGCGCAGCCTTCCCTCGCTGGTACT
ATGACACTGAAAAGAATTCCTGTATCAGCTTCATCTACGGCGGGTGCAGAGGCAACAAGAACAGTTACCT
CTCCCAGGAAGCATGCATGCAGCACTGCTCTGGTAAGCAGATGCATCCTTTCCTGACCCCGGGCCTAAAA
GCGGTGATCCTGGTGGGGCTGTTCCTCATGGTTCTGATCCTCCTCCTGGGAACCTCTATGGTCTGCCTCA
TCCGTGTGGTTCGCAGAAAGCAGGAGCGTGCCCTGCGCACTGTTTGGAGCACTGCGGATGACAAGGAGCA
GCTGGTGAAGAACACCTGTGTCTTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201901 protein sequence
Red=Cloning site Green=Tags(s)

MAQLCELRRGRALLALVASLLLSGAQVASRELDVHENTTDDMARNRNGADSSVLSVPRKQSAEDLSAEIF
NYEEYCVPKAVTGPCRAAFPRWYYDTEKNSCISFIYGGCRGNKNSYLSQEACMQHCSGKQMHPFLTPGLK
AVILVGLFLMVLILLLGTSMVCLIRVVRRKQERALRTVWSTADDKEQLVKNTCVL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001082548
ORF Size 585 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001082548.1, NP_001076017.1
RefSeq Size 1306 bp
RefSeq ORF 588 bp
Locus ID 20733
UniProt ID Q9WU03
Cytogenetics 7 B1
MW 21.7 kDa
Summary Inhibitor of HGF activator.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001082548" in other vectors (3)
SKU Description Size Price
MG201901 Spint2 (tGFP-tagged) - Mouse serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2 10 ug
$500.00
MR201901L3 Lenti ORF clone of Spint2 (Myc-DDK-tagged) - Mouse serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2 10 ug
$600.00
MR201901L4 Lenti ORF clone of Spint2 (mGFP-tagged) - Mouse serine protease inhibitor, Kunitz type 2 (Spint2), transcript variant 2 10 ug
$600.00

Plasmid Map for MR201901

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file-download TXT (FASTA format)

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Cloning scheme for MR201901
Circular map for MR201901

file-alt Citations

The catalytic, stem, and transmembrane portions of matriptase-2 are required for suppressing the expression of the iron-regulatory hormone hepcidin
Mao, Wortham, Enns et al
J. Biol. Chem. (2018) 294 (6), 2060-2073 DOI: 10.1074/jbc.RA118.006468
AI Summary: OriGene contributed to this study by providing the murine HAI-2 (NM_001082548) ORF with a C-terminal FLAG/MYC epitope in the pCMV6 vector, which was used to investigate the molecular mechanisms underlying MT2 function.
Snippet: … Murine HAI-2 (NM_001082548) ORF with a C-terminal FLAG/MYC epitope in pCMV6 vector was purchased;All mouse MT2, ALK2, ALK3, ActRIIA, Bmpr2, Hfe, β2-microglobulin (B2M), Hjv, Tfr2, and pEGFP-N1-expressing constructs were the same as previously described (9). Murine HAI-2 (NM_001082548) ORF with a C-terminal FLAG/MYC epitope in pCMV6 vector was purchased from Origene (Table 1). …
Showing 1-1 of 1 papers.
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