0610037D15Rik (BC022733) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201874
0610037D15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610037D15 gene (cDNA clone MGC:31599 IMAGE:4506628)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 0610037D15Rik
Synonyms 0610037D15Rik; 4933430J04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201874 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACAGGAGCCCTAGCTGGTCTGATCAGCTTCATGCGGTTCTCAGTTCAACTGATGAAAATATGGCCC
GGATTAAGAGTTTGTATCCCTTTCGAGTTTCCTCTACAGGGGACCTGGCTGACACCTGGACTTCTCATCG
CTTACCTCCTCAGCCAGAGGCCCAAGCTCAACAGCCTTGGGCTCTAAAGACTCCCACTCTTGGAGAGAGA
CTGAGCTGTACTGAAGCCCACAGCTCCTCTCTCTGGGATGAAGTTACTAAACTCCGATCCCAGCTTCAGG
CTCAGGCTCAGGTGACTGAGGCGCTGAAGCATTCTGTACAGAGCCTGTTGAAGGATCGGGAGCAACATAT
GTTCAAGATCAGTGCTCTAGAAGCATCACTAAAACTGCTGCAGGAGGGCCCCAAAGGGAGAGCCCTTCTG
GAGCAATGCCTGGAAGGGCTGAAAAAGGAACTGCAGGGCCTTCGGAGTCAGGTTCAAGAGCTAGTCCAAA
ACCAAATGAAAGCAAGACCAGGAAAGTTGGGTCCTGCCAGTGGCTTTCACCAAGAGCTGCAGAGTGAAAT
GACACTGCTGTCACTCATG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201874 protein sequence
Red=Cloning site Green=Tags(s)

MNRSPSWSDQLHAVLSSTDENMARIKSLYPFRVSSTGDLADTWTSHRLPPQPEAQAQQPWALKTPTLGER
LSCTEAHSSSLWDEVTKLRSQLQAQAQVTEALKHSVQSLLKDREQHMFKISALEASLKLLQEGPKGRALL
EQCLEGLKKELQGLRSQVQELVQNQMKARPGKLGPASGFHQELQSEMTLLSLM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC022733
ORF Size 579 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC022733, AAH22733
RefSeq Size 1251 bp
RefSeq ORF 581 bp
Locus ID 68394
Cytogenetics 4 D1
MW 21.6 kDa
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Documents
Resources
"BC022733" in other vectors (4)
SKU Description Size Price
MC206922 0610037D15Rik (untagged) - Mouse RIKEN cDNA 0610037D15 gene (cDNA clone MGC:31599 IMAGE:4506628), (10ug) 10 ug
$330.00
MG201874 0610037D15Rik (tGFP-tagged) - Mouse RIKEN cDNA 0610037D15 gene (cDNA clone MGC:31599 IMAGE:4506628) 10 ug
$500.00
MR201874L3 Lenti ORF clone of 0610037D15Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610037D15 gene (cDNA clone MGC:31599 IMAGE:4506628) 10 ug
$600.00
MR201874L4 Lenti ORF clone of 0610037D15Rik (mGFP-tagged) - Mouse RIKEN cDNA 0610037D15 gene (cDNA clone MGC:31599 IMAGE:4506628) 10 ug
$600.00

Plasmid Map for MR201874

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Cloning scheme for MR201874

Circular map for MR201874

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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