Sec11c (NM_025468) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201853
Sec11c (Myc-DDK-tagged) - Mouse SEC11 homolog C (S. cerevisiae) (Sec11c)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sec11c
Synonyms 1810029G24Rik; Sec11l3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201853 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTCGTGCGGGCGCCGTGGGGACGCATCTCCCCACGTCCAGCCTGGACATCTTTGGGGACCTGAGGA
AGATGAACAAACGACAGCTCTACTACCAGGTTTTAAACTTTGCCATGATCGTGTCTTCTGCGCTCATGAT
CTGGAAAGGCCTGATTGTTCTCACGGGCAGCGAGAGTCCCATCGTGGTGGTACTCAGTGGCAGTATGGAG
CCGGCCTTCCACAGAGGAGATCTGCTGTTCCTCACGAATTTCCGGGAGGACCCCATCAGAGCTGGTGAAA
TAGTTGTTTTTAAGGTTGAAGGAAGAGACATTCCGATAGTTCACAGAGTAATCAAGGTTCATGAAAAAGA
TAATGGTGACATCAAGTTTCTGACTAAAGGAGATAATAATGAAGTCGATGATAGAGGCTTGTACAAAGAA
GGCCAGAACTGGCTGGAGAAGAAGGACGTGGTAGGAAGAGCTCGAGGGTTCTTACCTTATGTTGGCATGG
TCACCATCATAATGAATGACTATCCAAAATTCAAGTATGCTCTTTTGGCTGTGATGGGTGCATATGTGTT
ACTAAAACGTGAATCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201853 protein sequence
Red=Cloning site Green=Tags(s)

MVRAGAVGTHLPTSSLDIFGDLRKMNKRQLYYQVLNFAMIVSSALMIWKGLIVLTGSESPIVVVLSGSME
PAFHRGDLLFLTNFREDPIRAGEIVVFKVEGRDIPIVHRVIKVHEKDNGDIKFLTKGDNNEVDDRGLYKE
GQNWLEKKDVVGRARGFLPYVGMVTIIMNDYPKFKYALLAVMGAYVLLKRES

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025468
ORF Size 576 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025468.2
RefSeq Size 770 bp
RefSeq ORF 579 bp
Locus ID 66286
UniProt ID Q9D8V7
Cytogenetics 18 E1
MW 21.7 kDa
Summary Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum.UniProtKB/Swiss-Prot Function
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"NM_025468" in other vectors (4)
SKU Description Size Price
MC203063 Sec11c (untagged) - Mouse SEC11 homolog C (S. cerevisiae) (Sec11c), (10ug) 10 ug
$300.00
MG201853 Sec11c (tGFP-tagged) - Mouse SEC11 homolog C (S. cerevisiae) (Sec11c) 10 ug
$500.00
MR201853L3 Lenti ORF clone of Sec11c (Myc-DDK-tagged) - Mouse SEC11 homolog C (S. cerevisiae) (Sec11c) 10 ug
$600.00
MR201853L4 Lenti ORF clone of Sec11c (mGFP-tagged) - Mouse SEC11 homolog C (S. cerevisiae) (Sec11c) 10 ug
$600.00

Plasmid Map for MR201853

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Cloning scheme for MR201853

Circular map for MR201853

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