Rhog (NM_019566) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201818
Rhog (Myc-DDK-tagged) - Mouse ras homolog gene family, member G (Rhog)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhog
Synonyms 2810426G09Rik; Arhg; Sid10750
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201818 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGCATCAAGTGTGTGGTGGTGGGCGATGGGGCCGTAGGCAAGACGTGCCTTCTCATCTGCTATA
CAACTAACGCCTTCCCCAAGGAATACATCCCCACTGTGTTTGACAATTACAGCGCCCAGAGTGCGGTTGA
TGGGCGCACCGTGAACCTAAACCTGTGGGACACTGCGGGCCAGGAGGAATATGACCGCCTCCGCACCCTT
TCCTACCCTCAGACCAACGTCTTCGTCATCTGTTTCTCCATTGCCAGTCCACCCTCCTATGAGAACGTGA
GGCACAAATGGCACCCAGAGGTTTGTCACCACTGCCCTGACGTGCCTATCCTCCTGGTGGGTACCAAGAA
GGACCTGAGAGCCCAGCCTGATACCCTACGGCGCCTCAAGGAGCAGGGTCAAGCGCCCATCACTCCACAG
CAGGGCCAGGCACTGGCCAAGCAGATCCATGCTGTGCGCTACCTCGAGTGCTCAGCACTGCAACAAGACG
GCGTCAAGGAGGTGTTTGCGGAGGCTGTCCGGGCGGTGCTTAACCCAACACCGATAAAGCGTGGGCGGTC
CTGCATCCTCTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201818 protein sequence
Red=Cloning site Green=Tags(s)

MQSIKCVVVGDGAVGKTCLLICYTTNAFPKEYIPTVFDNYSAQSAVDGRTVNLNLWDTAGQEEYDRLRTL
SYPQTNVFVICFSIASPPSYENVRHKWHPEVCHHCPDVPILLVGTKKDLRAQPDTLRRLKEQGQAPITPQ
QGQALAKQIHAVRYLECSALQQDGVKEVFAEAVRAVLNPTPIKRGRSCILL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019566
ORF Size 573 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019566.3
RefSeq Size 1264 bp
RefSeq ORF 576 bp
Locus ID 56212
UniProt ID P84096
Cytogenetics 7 E2
MW 21.3 kDa
Summary Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_019566" in other vectors (4)
SKU Description Size Price
MC204635 Rhog (untagged) - Mouse ras homolog gene family, member G (Rhog), (10ug) 10 ug
$300.00
MG201818 Rhog (tGFP-tagged) - Mouse ras homolog gene family, member G (Rhog) 10 ug
$500.00
MR201818L3 Lenti ORF clone of Rhog (Myc-DDK-tagged) - Mouse ras homolog gene family, member G (Rhog) 10 ug
$600.00
MR201818L4 Lenti ORF clone of Rhog (mGFP-tagged) - Mouse ras homolog gene family, member G (Rhog) 10 ug
$600.00

Plasmid Map for MR201818

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Cloning scheme for MR201818

Circular map for MR201818

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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