Slc25a37 (BC040399) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201755
Slc25a37 (Myc-DDK-tagged) - Mouse solute carrier family 25, member 37 (cDNA clone MGC:28425 IMAGE:4037680)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc25a37
Synonyms 1700020E22Rik; 4930513O14Rik; 4930526G11Rik; AI848481; C330015G08Rik; frascati; Mfrn; Mfrn1; mitoferrin; Mscp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201755 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACCCTACTCCACGATGCAGTAATGAATCCAGCAGAAGTGGTGAAACAGCGCTTACAGATGTACA
ACTCCCAGCACCAGTCAGCCTTCAGTTGTATCCGGACAGTGTGGCGGACCGAGGGGTTGGGGGCCTTCTA
CAGGAGTTACACCACACAGCTGACCATGAATATCCCCTTCCAGTCAATTCACTTCATCACCTATGAGTTT
CTGCAGGAGCAAGTCAACCCTCGCCGGGACTACAACCCACAGTCTCACATCATCTCAGGAGGCCTGGCCG
GGGCACTGGCCGCAGCTGCCACCACCCCGCTGGACGTCTGCAAAACCCTCCTCAACACGCAGGAGAACAT
GGCTCTCTCCCTGGCCAACGTCAGCGGCCGGCTGTCGGGCATGGCCAATGCCTTCCGGACGGTGTACCAG
CTCAACGGCCTTGCCGGCTATTTCAAAGGCATCCAGGCTCGAGTCATCTACCAGATGCCTTCCACCGCCA
TCTCCTGGTCCGTTTATGAGTTCTTCAAGTACATCCTTACAAAGAGGCAGCTGGAGAATCGAACTCTGTA
C


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201755 protein sequence
Red=Cloning site Green=Tags(s)

MATLLHDAVMNPAEVVKQRLQMYNSQHQSAFSCIRTVWRTEGLGAFYRSYTTQLTMNIPFQSIHFITYEF
LQEQVNPRRDYNPQSHIISGGLAGALAAAATTPLDVCKTLLNTQENMALSLANVSGRLSGMANAFRTVYQ
LNGLAGYFKGIQARVIYQMPSTAISWSVYEFFKYILTKRQLENRTLY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC040399
ORF Size 561 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC040399, AAH40399
RefSeq Size 4000 bp
RefSeq ORF 563 bp
Locus ID 67712
Cytogenetics 14 D2
MW 21.2 kDa
Summary Mitochondrial iron transporter that specifically mediates iron uptake in developing erythroid cells, thereby playing an essential role in heme biosynthesis. The iron delivered into the mitochondria, presumably as Fe(2+), is then probably delivered to ferrochelatase to catalyze Fe(2+) incorporation into protoprophyrin IX to make heme.UniProtKB/Swiss-Prot Function
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Documents
Resources
"BC040399" in other vectors (4)
SKU Description Size Price
MC207102 Slc25a37 (untagged) - Mouse solute carrier family 25, member 37 (cDNA clone MGC:28425 IMAGE:4037680), (10ug) 10 ug
$330.00
MG201755 Slc25a37 (tGFP-tagged) - Mouse solute carrier family 25, member 37 (cDNA clone MGC:28425 IMAGE:4037680) 10 ug
$500.00
MR201755L3 Lenti ORF clone of Slc25a37 (Myc-DDK-tagged) - Mouse solute carrier family 25, member 37 (cDNA clone MGC:28425 IMAGE:4037680) 10 ug
$600.00
MR201755L4 Lenti ORF clone of Slc25a37 (mGFP-tagged) - Mouse solute carrier family 25, member 37 (cDNA clone MGC:28425 IMAGE:4037680) 10 ug
$600.00

Plasmid Map for MR201755

Download sequence for

Cloning scheme for MR201755

Circular map for MR201755

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