OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Nme4 (NM_019731) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR201741
Nme4 (Myc-DDK-tagged) - Mouse non-metastatic cells 4, protein expressed in (Nme4), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Nme4 or NM_019731"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nme4
Synonyms 2610027N22Rik; 2810024O08Rik; 5730493H09Rik; NM23-M4; Nm23M4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201741 representing NM_019731
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAGCCTTTTCGGGCGCGTCGCGGCGCTGCGGGCGCTGTTGTGCGGGCCACGCTTTCAGTGCCTGC
TGGTGCGCCCCAGTTCCGGAGGCCCCCCCTGGCCCCAAGAGCGGACGCTGGTTGCTGTGAAGCCAGATGG
GGTACAGAGGAGACTAGTGGGGACTGTGATACAACGCTTTGAGAGGCGGGGCTTCAAGCTCGTGGGGATG
AAGATGCTGCAGGCACCAGAAAGCATCCTTGCTGAGCACTACCGGGACCTACAGAGGAAGCCATTCTACC
CAGCTCTTATCAGCTACATGAGCTCTGGGCCTGTGGTGGCCATGGTCTGGGAAGGCCCCAATGTGGTCCA
TATCTCAAGGGCCATGATAGGACACACCGACTCAACAGAGGCAGCCCCCGGGACAATCAGGGGCGACTTC
AGTGTTCACATCAGCAGGAACGTCATCCATGCTAGCGATTCTGTGGATGGGGCCCAGAGGGAGATCGAGC
TGTGGTTTCAGAGCAGCGAACTGTTGAACTGGGCAGATGGTGGTCACCACAGCAGCTGCTACCCTGCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201741 representing NM_019731
Red=Cloning site Green=Tags(s)

MGSLFGRVAALRALLCGPRFQCLLVRPSSGGPPWPQERTLVAVKPDGVQRRLVGTVIQRFERRGFKLVGM
KMLQAPESILAEHYRDLQRKPFYPALISYMSSGPVVAMVWEGPNVVHISRAMIGHTDSTEAAPGTIRGDF
SVHISRNVIHASDSVDGAQREIELWFQSSELLNWADGGHHSSCYPA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019731
ORF Size 558 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019731.1, NP_062705.1
RefSeq Size 863 bp
RefSeq ORF 561 bp
Locus ID 56520
UniProt ID Q9WV84
Cytogenetics 17 A3.3
MW 21 kDa
Summary Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Through the catalyzed exchange of gamma-phosphate between di- and triphosphonucleosides participates in regulation of intracellular nucleotide homeostasis. Binds to anionic phospholipids, predominantly to cardiolipin; the binding inhibits its phosphotransfer activity. Acts as mitochondria-specific NDK; its association with cardiolipin-containing mitochondrial inner membrane is coupled to respiration suggesting that ADP locally regenerated in the mitochondrion innermembrane space by its activity is directly taken up via ANT ADP/ATP translocase into the matrix space to stimulate respiratory ATP regeneration. Proposed to increase GTP-loading on dynamin-related GTPase OPA1 in mitochondria. In vitro can induce liposome cross-linking suggesting that it can cross-link inner and outer membranes to form contact sites, and promotes intermembrane migration of anionic phosphoplipids. Promotes the redistribution of cardiolipin between the mitochondrial inner membrane and outer membrane which is implicated in pro-apoptotic signaling (By similarity).UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_019731" in other vectors (4)
SKU Description Size Price
MC201533 Nme4 (untagged) - Mouse non-metastatic cells 4, protein expressed in (Nme4), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG201741 Nme4 (tGFP-tagged) - Mouse expressed in non-metastatic cells 4, protein (Nme4) 10 ug
$500.00
MR201741L3 Lenti ORF clone of Nme4 (Myc-DDK-tagged) - Mouse non-metastatic cells 4, protein expressed in (Nme4), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR201741L4 Lenti ORF clone of Nme4 (mGFP-tagged) - Mouse non-metastatic cells 4, protein expressed in (Nme4), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR201741

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201741
Circular map for MR201741

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products