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Ndufaf3 (NM_023247) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201729
Ndufaf3 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 (Ndufaf3), nuclear gene encoding mitochondrial protein
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ndufaf3 or NM_023247"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ndufaf3
Synonyms 4733401H18Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201729 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTACTGCTCTAGGATTTCGCTGCCTTTTCCGAACGCGACCTGCGCCGCTCTGTCGGCACGTCGACC
GGCTGTGGAGGAACCCGCGTCGGGGACACCGGCTTTCTCCGGCGGACGATGAGTTATACCAGCGGACTCG
CATCTCACTGCTGCAAAGCGAGTTCCCTCAGGCCGTGTACATCGACAGCTACAGTAGCAGAGGCTTCACT
ATCTGCGGAAATCGCGTGTTTGGCCCTTGCGTGCTGCTCCCACAGACGGTGGTCCATTGGAACGTGGGAT
CTCATCAGGATATCACTGAAGAGAGCTTCTCCCTTTTCTGGATGCTGGAACCTAGGATAGAGATTGTTGT
GGTGGGCACTGGAAACAAGACTGAGCGGCTGCACCCTCAAGTACTACAGGCCATGAGGCAGCGGGGAATT
GCTGTGGAGGTCCAGGACACGCCCAATGCCTGTGCCACTTTCAACTTCCTGTGCCACGAAGGTCGAGTGA
CAGGAGCTGCCCTCATTCCCCCTCCTGGAGAGACTGCACTGGCTTCTTTGGGTCAAGCCTCAGAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201729 protein sequence
Red=Cloning site Green=Tags(s)

MATALGFRCLFRTRPAPLCRHVDRLWRNPRRGHRLSPADDELYQRTRISLLQSEFPQAVYIDSYSSRGFT
ICGNRVFGPCVLLPQTVVHWNVGSHQDITEESFSLFWMLEPRIEIVVVGTGNKTERLHPQVLQAMRQRGI
AVEVQDTPNACATFNFLCHEGRVTGAALIPPPGETALASLGQASE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023247
ORF Size 555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023247.1, NP_075736.1
RefSeq Size 1092 bp
RefSeq ORF 558 bp
Locus ID 66706
UniProt ID Q9JKL4
Cytogenetics 9 F2
MW 20.7 kDa
Summary Essential factor for the assembly of mitochondrial NADH:ubiquinone oxidoreductase complex (complex I).UniProtKB/Swiss-Prot Function
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Documents
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"NM_023247" in other vectors (4)
SKU Description Size Price
MC204665 Ndufaf3 (untagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 (Ndufaf3), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$300.00
MG201729 Ndufaf3 (tGFP-tagged) - Mouse RIKEN cDNA 4733401H18 gene (4733401H18Rik) 10 ug
$500.00
MR201729L3 Lenti ORF clone of Ndufaf3 (Myc-DDK-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 (Ndufaf3), nuclear gene encoding mitochondrial protein 10 ug
$600.00
MR201729L4 Lenti ORF clone of Ndufaf3 (mGFP-tagged) - Mouse NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 3 (Ndufaf3), nuclear gene encoding mitochondrial protein 10 ug
$600.00

Plasmid Map for MR201729

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201729
Circular map for MR201729

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