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Tmem140 (NM_197986) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201714
Tmem140 (Myc-DDK-tagged) - Mouse transmembrane protein 140 (Tmem140)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem140
Synonyms 1110007F12Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201714 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTCTCCAGGCTGTGGCGGAACAACCACCTGCCCTTCGTGGGCATCATGATCCTCTTGGCCGCGG
CCCTGTGCCTGATGTTCTACGCTCTCCTCTGGAAGGCTGGCAACCTCGCTGACCTACCTAGCCTGAGAAT
CGGCTTCTACAACTTCTGTCTGTGGAAGGAGGACATGGGCTCCCTAGCATGTTACAACTTTCCCGAGCTG
GACGTGCTGGGCATTCCTCAGGTTGGACTAGCCCTGGCCAGGCTCGGTGTGTATGGAGCCCTGGTCCTCA
CAGTGTTCGTCCCTCTGCCTCTCCTCCTTGCCCAGTACAACAGAGATGAGGGAGAGTGGCGGCTGGCCGT
GTGCTTCCTGGCGGCATCCTCCATTCTGTTGGCCGGAGGACTAAGCCTCTTCCTCTCCTTCGTGTGGAAG
TGGCTCAGGCTCTCCTTCCTGGGGCCTGCCTTGCCAGCTCTATGCCTAGCCCAGCTGTTACTCATCTTCT
TGCTTGTGGCCACGGTTAGGTTCCCGCCACGGGATAAGGAGGACAAGAACCAGTGGGAGAACTGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201714 protein sequence
Red=Cloning site Green=Tags(s)

MAFSRLWRNNHLPFVGIMILLAAALCLMFYALLWKAGNLADLPSLRIGFYNFCLWKEDMGSLACYNFPEL
DVLGIPQVGLALARLGVYGALVLTVFVPLPLLLAQYNRDEGEWRLAVCFLAASSILLAGGLSLFLSFVWK
WLRLSFLGPALPALCLAQLLLIFLLVATVRFPPRDKEDKNQWENC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_197986
ORF Size 555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_197986.3
RefSeq Size 2614 bp
RefSeq ORF 558 bp
Locus ID 68487
UniProt ID Q8BGY5
Cytogenetics 6 B1
MW 20.8 kDa
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"NM_197986" in other vectors (4)
SKU Description Size Price
MC201730 Tmem140 (untagged) - Mouse transmembrane protein 140 (Tmem140), (10ug) 10 ug
$300.00
MG201714 Tmem140 (tGFP-tagged) - Mouse transmembrane protein 140 (Tmem140) 10 ug
$500.00
MR201714L3 Lenti ORF clone of Tmem140 (Myc-DDK-tagged) - Mouse transmembrane protein 140 (Tmem140) 10 ug
$600.00
MR201714L4 Lenti ORF clone of Tmem140 (mGFP-tagged) - Mouse transmembrane protein 140 (Tmem140) 10 ug
$600.00

Plasmid Map for MR201714

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201714
Circular map for MR201714

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