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D2Wsu81e (BC078441) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201597
D2Wsu81e (Myc-DDK-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "D2Wsu81e or BC078441"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol D2Wsu81e
Synonyms 9930028P05; AL033358
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGAAACTGGAGCGGCAGCGTGCCCAAGAGGAAGAGGCTAAACGCCAGGAAGAGGAGGAAGAGGCGG
CGGCCCAGAGGAGCAACCAAGGACGGCCTTACACTCTGAGTGTGGCCCTGCCAGGCTCCATCCTGGACAA
TGCCCAGTCACCTGAGCTCCGCACCTACCTGGCTGGCCAGATCGCCAGAGCCTGCACCATCTTCTGTGTG
GATGAGATTGTGGTGTTCGATGAGGAGGGCCAAGATACCAAGAGTGTGGAAGGAGAATTCAGGGGAGTTG
GCAAGAAAGGGCAGGCATGTGTCCAGCTGGCCCGCATCCTACAGTACCTGGAGTGTCCACAGTACCTGAG
AAAGGCGTTCTTCCCCAAGCACCAAGATCTGCAGTTTGCAGGAATCCTCAACCCCTTGGACAGCCCTCAC
CACATGCGTCAGGACGAAGAGTCTGAGTTTCGAGAAGGCGTCGTGGTGGACCGGCCCACCAAGGCAGGCC
ATGGCTCTTTGGTCAACTGTGGAATGAAGAAGCTTCTCTTCAGGAGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201597 protein sequence
Red=Cloning site Green=Tags(s)

MKKLERQRAQEEEAKRQEEEEEAAAQRSNQGRPYTLSVALPGSILDNAQSPELRTYLAGQIARACTIFCV
DEIVVFDEEGQDTKSVEGEFRGVGKKGQACVQLARILQYLECPQYLRKAFFPKHQDLQFAGILNPLDSPH
HMRQDEESEFREGVVVDRPTKAGHGSLVNCGMKKLLFRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC078441
ORF Size 537 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC078441, AAH78441
RefSeq Size 2440 bp
RefSeq ORF 539 bp
Locus ID 227695
Cytogenetics 2 21.29 cM
MW 20.3 kDa
Summary Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis. Binds specifically to miRNA MIR145 hairpin, regulates MIR145 expression at a postranscriptional level (By similarity).UniProtKB/Swiss-Prot Function
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"BC078441" in other vectors (4)
SKU Description Size Price
MC206986 D2Wsu81e (untagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308), (10ug) 10 ug
$330.00
MG201597 D2Wsu81e (tGFP-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308) 10 ug
$500.00
MR201597L3 Lenti ORF clone of D2Wsu81e (Myc-DDK-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308) 10 ug
$600.00
MR201597L4 Lenti ORF clone of D2Wsu81e (mGFP-tagged) - Mouse DNA segment, Chr 2, Wayne State University 81, expressed (cDNA clone MGC:90552 IMAGE:5686308) 10 ug
$600.00

Plasmid Map for MR201597

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201597
Circular map for MR201597

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