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Tmem110 (BC024583) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201512
Tmem110 (Myc-DDK-tagged) - Mouse transmembrane protein 110 (cDNA clone MGC:37710 IMAGE:5065836)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tmem110 or BC024583"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tmem110
Synonyms 1810038N08Rik; 2310014H19Rik; AW554125; STIMATE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201512 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCTCATTTATGTGGGCGTACGCGCCGTCGGTGTCTTGGTGGAGTGGCAGCAGTGGGAATCCCTGC
GTTTTGGAGAATATGGAGACCCTCTGCAGTGCGGGGCCTGGGTAGGACAGTGCGCCCTCTACATCGTGAT
CATGATCTTTGAAAAGTCCGTGGTCTTCATCGTCCTCCTCATACTCCAATGGAAAAAGGTGGCCCTATTG
AATCCAATTGAAAACCCCGACCTGAAGCTGGCCATCGTCATGCTGATAGTCCCCTTCTTTGTCAATGCTT
TCATGTTCTGGGTAGTGGACAATTTCCTCATGAGAAAGGGGAAGACGAAAGCTAAACTAGAAGAAAGAGG
AGCCAACCAGGACTCAAGGAATGGGAGCAAGGTTCGCTACCGAAGGGCAGCATCCCATGAGGAGTCTGAG
TCTGAGATCCTGATCTCAGCTGACGACGAGATGGAGGAGTCTGACGCCGAGGAGGACCTCCGGAGACCTG
TGAAAAAGAAGCACCGCTTCGGGCTGCCTGTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201512 protein sequence
Red=Cloning site Green=Tags(s)

MLLIYVGVRAVGVLVEWQQWESLRFGEYGDPLQCGAWVGQCALYIVIMIFEKSVVFIVLLILQWKKVALL
NPIENPDLKLAIVMLIVPFFVNAFMFWVVDNFLMRKGKTKAKLEERGANQDSRNGSKVRYRRAASHEESE
SEILISADDEMEESDAEEDLRRPVKKKHRFGLPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC024583
ORF Size 522 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC024583, AAH24583
RefSeq Size 1196 bp
RefSeq ORF 524 bp
Locus ID 69179
Cytogenetics 14 B
MW 20.1 kDa
Summary Acts as a regulator of store-operated Ca(2+) entry (SOCE) at junctional sites that connect the endoplasmic reticulum (ER) and plasma membrane (PM), called ER-plasma membrane (ER-PM) junction or cortical ER. SOCE is a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts by interacting with STIM1, promoting STIM1 conformational switch. Involved in STIM1 relocalization to ER-PM junctions. Contributes to the maintenance and reorganization of store-dependent ER-PM junctions.UniProtKB/Swiss-Prot Function
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"BC024583" in other vectors (4)
SKU Description Size Price
MC206930 Tmem110 (untagged) - Mouse transmembrane protein 110 (cDNA clone MGC:37710 IMAGE:5065836), (10ug) 10 ug
$330.00
MG201512 Tmem110 (tGFP-tagged) - Mouse transmembrane protein 110 (cDNA clone MGC:37710 IMAGE:5065836) 10 ug
$500.00
MR201512L3 Lenti ORF clone of Tmem110 (Myc-DDK-tagged) - Mouse transmembrane protein 110 (cDNA clone MGC:37710 IMAGE:5065836) 10 ug
$600.00
MR201512L4 Lenti ORF clone of Tmem110 (mGFP-tagged) - Mouse transmembrane protein 110 (cDNA clone MGC:37710 IMAGE:5065836) 10 ug
$600.00

Plasmid Map for MR201512

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201512
Circular map for MR201512

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