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Cd99 (NM_025584) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201505
Cd99 (Myc-DDK-tagged) - Mouse CD99 antigen (Cd99)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $450.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cd99
Synonyms 1110061M03Rik; 2410026K10Rik; D4; pilr-1; Pilr-l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201505 representing NM_025584
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGCGCGCGGCGATGGAGGCGGCGGCGACCGTGGTTTTGGCCCTGGCGCTGCTCGGGGCCGCGGCGC
GGGGCGCGGCGAGTGACGACTTCAACCTGGGCGACGCCCTGGAGGACCCCAACATGAAGCCAACCCCCAA
GGCCCCAACGCCCAAGAAGCCGTCAGGAGGCTTCGACCTGGAGGACGCCCTGCCCGGCGGCGGCGGAGGA
GGCGCAGGAGAGAAGCCGGGAAACCGGCCCCAGCCGGACCCGAAGCCGCCAAGGCCACACGGAGACTCAG
GCGGCATCTCGGACAGCGACCTGGCAGACGCCGCGGGCCAGGGAGGAGGCGGAGCCGGGCGACGCGGGAG
CGGAGACGAAGGCGGCCATGGCGGCGCTGGCGGGGCGGAGCCGGAAGGGACGCCCCAGGGCTTGGTGCCG
GGCGTGGTGGCGGCCGTGGTGGCCGCCGTGGCGGGCGCGGTGTCGAGCTTCGTGGCCTATCAGCGGCGGC
GCCTGTGCTTCCGCGAGGGCGGCTCCGCCCCCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201505 representing NM_025584
Red=Cloning site Green=Tags(s)

MARAAMEAAATVVLALALLGAAARGAASDDFNLGDALEDPNMKPTPKAPTPKKPSGGFDLEDALPGGGGG
GAGEKPGNRPQPDPKPPRPHGDSGGISDSDLADAAGQGGGGAGRRGSGDEGGHGGAGGAEPEGTPQGLVP
GVVAAVVAAVAGAVSSFVAYQRRRLCFREGGSAPV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025584
ORF Size 525 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025584.2, NP_079860.2
RefSeq Size 950 bp
RefSeq ORF 528 bp
Locus ID 673094
UniProt ID Q8VCN6
Cytogenetics 4
MW 17.2 kDa
Summary Involved in T-cell adhesion processes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1.UniProtKB/Swiss-Prot Function
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"NM_025584" in other vectors (4)
SKU Description Size Price
MC203982 Cd99 (untagged) - Mouse CD99 antigen (Cd99), (10ug) 10 ug
$450.00
MG201505 Cd99 (tGFP-tagged) - Mouse CD99 antigen (Cd99) 10 ug
$650.00
MR201505L3 Lenti ORF clone of Cd99 (Myc-DDK-tagged) - Mouse CD99 antigen (Cd99) 10 ug
$750.00
MR201505L4 Lenti ORF clone of Cd99 (mGFP-tagged) - Mouse CD99 antigen (Cd99) 10 ug
$750.00

Plasmid Map for MR201505

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201505
Circular map for MR201505

file-alt Citations

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