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Cd3d (NM_013487) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201480
Cd3d (Myc-DDK-tagged) - Mouse CD3 antigen, delta polypeptide (Cd3d)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cd3d
Synonyms T3d
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201480 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAACACAGCGGGATTCTGGCTAGTCTGATACTGATTGCTGTTCTCCCCCAAGGGAGCCCCTTCAAGG
TACAAGTGACCGAATATGAGGACAAAGTATTTGTGACCTGCAATACCAGCGTCATGCATCTAGATGGAAC
GGTGGAAGGATGGTTTGCAAAGAATAAAACACTCAACTTGGGCAAAGGCGTTCTGGACCCACGAGGGATA
TATCTGTGTAATGGGACAGAGCAGCTGGCAAAGGTGGTGTCTTCTGTGCAAGTCCATTACCGAATGTGCC
AGAACTGTGTGGAGCTAGACTCGGGCACCATGGCTGGTGTCATCTTCATTGACCTCATCGCAACTCTGCT
CCTGGCTTTGGGCGTCTACTGCTTTGCAGGACATGAGACCGGAAGGCCTTCTGGGGCTGCTGAGGTTCAA
GCACTGCTGAAGAATGAGCAGCTGTATCAGCCTCTTCGAGATCGTGAAGATACCCAGTACAGCCGTCTTG
GAGGGAACTGGCCCCGGAACAAGAAATCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201480 protein sequence
Red=Cloning site Green=Tags(s)

MEHSGILASLILIAVLPQGSPFKVQVTEYEDKVFVTCNTSVMHLDGTVEGWFAKNKTLNLGKGVLDPRGI
YLCNGTEQLAKVVSSVQVHYRMCQNCVELDSGTMAGVIFIDLIATLLLALGVYCFAGHETGRPSGAAEVQ
ALLKNEQLYQPLRDREDTQYSRLGGNWPRNKKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013487
ORF Size 519 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013487.2, NP_038515.2
RefSeq Size 1330 bp
RefSeq ORF 522 bp
Locus ID 12500
UniProt ID P04235
Cytogenetics 9 24.84 cM
MW 19 kDa
Summary Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly (PubMed:10935641). Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells.UniProtKB/Swiss-Prot Function
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"NM_013487" in other vectors (4)
SKU Description Size Price
MC207244 Cd3d (untagged) - Mouse CD3 antigen, delta polypeptide (Cd3d), (10ug) 10 ug
$330.00
MG201480 Cd3d (tGFP-tagged) - Mouse CD3 antigen, delta polypeptide (Cd3d) 10 ug
$500.00
MR201480L3 Lenti ORF clone of Cd3d (Myc-DDK-tagged) - Mouse CD3 antigen, delta polypeptide (Cd3d) 10 ug
$600.00
MR201480L4 Lenti ORF clone of Cd3d (mGFP-tagged) - Mouse CD3 antigen, delta polypeptide (Cd3d) 10 ug
$600.00

Plasmid Map for MR201480

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201480
Circular map for MR201480

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