Fuom (NM_026928) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201413
Fuom (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810014F10 gene (1810014F10Rik)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fuom
Synonyms Fucu; Le51
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201413 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTTGCGCTCAAGGGCATCCCGAAGGTGCTGTCTCCTGAGCTGTTGTTCGCGCTTGCGCGGATGGGGC
ATGGAGACGAAATTGTCCTTGCTGATGCGAACTTCCCTACCTCCTCCATCTGCCAGTGCGGACCTGTGGA
GATCCGAGCAGACGGCCTGGACATCCCACAGCTTCTGGAGGCTGTGCTGAGGCTCCTGCCCCTGGACACC
TACGTGGAAAGCCCGGCTGCTGTCATGGACCTGGTGCCCAGTGACAAGGAGAAGGGCCTGCAGACCCCGA
TATGGAAGCGTTATGAATCCCTTCTTCTCGAAGCTGACTGTAAAAAAACCCTGATGAAGCTAGAGAGATT
TGAATTTTATGAACGTGCAAAAAAGGCATTTGCTGTGGTTGCAACCGGGGAGATGGCACTCTATGGAAAC
ATCATCCTCAAGAAGGGAACTCTTGACCTCGGACCCTCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201413 protein sequence
Red=Cloning site Green=Tags(s)

MVALKGIPKVLSPELLFALARMGHGDEIVLADANFPTSSICQCGPVEIRADGLDIPQLLEAVLRLLPLDT
YVESPAAVMDLVPSDKEKGLQTPIWKRYESLLLEADCKKTLMKLERFEFYERAKKAFAVVATGEMALYGN
IILKKGTLDLGPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026928
ORF Size 459 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026928.4
RefSeq Size 2340 bp
RefSeq ORF 462 bp
Locus ID 69064
UniProt ID Q8R2K1
Cytogenetics 7 F4
MW 16.8 kDa
Summary Involved in the interconversion between alpha- and beta-L-fucoses. L-Fucose (6-deoxy-L-galactose) exists as alpha-L-fucose (29.5%) and beta-L-fucose (70.5%), the beta-form is metabolized through the salvage pathway. GDP-L-fucose formed either by the de novo or salvage pathways is transported into the endoplasmic reticulum, where it serves as a substrate for N- and O-glycosylations by fucosyltransferases. Fucosylated structures expressed on cell surfaces or secreted in biological fluids are believed to play a critical role in cell-cell adhesion and recognition processes.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026928" in other vectors (4)
SKU Description Size Price
MC201372 Fuom (untagged) - Mouse RIKEN cDNA 1810014F10 gene (1810014F10Rik), (10ug) 10 ug
$150.00
MG201413 Fuom (tGFP-tagged) - Mouse RIKEN cDNA 1810014F10 gene (1810014F10Rik) 10 ug
$350.00
MR201413L3 Lenti ORF clone of Fuom (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810014F10 gene (1810014F10Rik) 10 ug
$450.00
MR201413L4 Lenti ORF clone of Fuom (mGFP-tagged) - Mouse RIKEN cDNA 1810014F10 gene (1810014F10Rik) 10 ug
$450.00

Plasmid Map for MR201413

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Cloning scheme for MR201413

Circular map for MR201413

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