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Gtf3a (BC032292) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201406
Gtf3a (Myc-DDK-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Gtf3a or BC032292"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gtf3a
Synonyms 2010015D03Rik; 2610111I01Rik; 5330403M05Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201406 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAAAACGTGGACAGAGCTTCTGAAACACATGAGAGAAGCCCATAAAGAGGACATAACCTGCAACG
TATGTCAGAGGATGTTCAAGCGCAGAGATTACCTTAAGCAGCACATGAAGACTCACGCCCCGGAAAGGGA
TGTGTACCGCTGTCCGCGGCAAGGCTGCGGAAGAACCTACACAACCGTGTTCAACCTGCAGAGCCACATT
CTCTCCTTCCACGAGGAAAAGCGCCCATTTGTGTGTGAGCACGCTGGCTGTGGCAAGACATTCGCAATGA
AACAGAGTCTCATGAGGCACAGTGTCGTGCACGATCCCGACAAGAAGAGGATGAAGCTCAAAGTAAGAGC
CCCTCGGGAGAGACGCAGCTTGGCCTCTCGCCTCAGTGGGTACTTCCCTCCTAAGAGGAAACAAGAGCCC
GACTACTCCTTGCCTAACGCCAGCGCAGAGTCCAGCAGCAGTCCAGAGGCCCAGCTGCCCCCGCCAGCCA
CCTTACTCACTGTCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201406 protein sequence
Red=Cloning site Green=Tags(s)

MGKTWTELLKHMREAHKEDITCNVCQRMFKRRDYLKQHMKTHAPERDVYRCPRQGCGRTYTTVFNLQSHI
LSFHEEKRPFVCEHAGCGKTFAMKQSLMRHSVVHDPDKKRMKLKVRAPRERRSLASRLSGYFPPKRKQEP
DYSLPNASAESSSSPEAQLPPPATLLTVC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC032292
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC032292, AAH32292
RefSeq Size 996 bp
RefSeq ORF 509 bp
Locus ID 66596
Cytogenetics 5 G3
MW 19.7 kDa
Summary The product of this gene is a zinc finger protein with nine Cis2-His2 zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR), and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis, protein homology, and the size of the purified protein. provided by RefSeq, Jul 2008
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"BC032292" in other vectors (3)
SKU Description Size Price
MG201406 Gtf3a (tGFP-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268) 10 ug
$500.00
MR201406L3 Lenti ORF clone of Gtf3a (Myc-DDK-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268) 10 ug
$600.00
MR201406L4 Lenti ORF clone of Gtf3a (mGFP-tagged) - Mouse general transcription factor III A (cDNA clone MGC:40923 IMAGE:5374268) 10 ug
$600.00

Plasmid Map for MR201406

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201406
Circular map for MR201406

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