Ptp4a2 (NM_008974) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201376
Ptp4a2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase 4a2 (Ptp4a2), transcript variant 1
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptp4a2
Synonyms Prl-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201376 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACCGTCCAGCCCCTGTGGAGATCTCTTATGAGAACATGCGTTTTCTGATAACTCACAACCCCACCA
ATGCGACTCTCAACAAGTTCACAGAGGAACTTAAGAAGTACGGAGTGACAACTTTGGTTCGAGTTTGTGA
TGCTACATATGATAAAGCTCCAGTTGAAAAAGAAGGAATCCACGTTCTAGATTGGCCGTTTGATGATGGA
GCTCCACCCCCTAATCAGATAGTAGATGATTGGCTAAACCTGTTAAAAACCAAATTTCGTGAAGAGCCAG
GCTGTTGTGTTGCAGTGCATTGTGTTGCAGGATTGGGAAGGGCTCCTGTGCTAGTTGCGCTTGCATTGAT
TGAATGCGGAATGAAGTATGAAGATGCTGTTCAATTTATAAGACAAAAAAGAAGAGGAGCATTCAATTCC
AAACAGCTGCTTTACTTGGAGAAGTACCGACCTAAGATGCGGTTACGCTTCAGAGATACCAATGGGCACT
GCTGTGTTCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201376 protein sequence
Red=Cloning site Green=Tags(s)

MNRPAPVEISYENMRFLITHNPTNATLNKFTEELKKYGVTTLVRVCDATYDKAPVEKEGIHVLDWPFDDG
APPPNQIVDDWLNLLKTKFREEPGCCVAVHCVAGLGRAPVLVALALIECGMKYEDAVQFIRQKRRGAFNS
KQLLYLEKYRPKMRLRFRDTNGHCCVQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008974
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008974.4, NP_033000.1
RefSeq Size 3413 bp
RefSeq ORF 504 bp
Locus ID 19244
UniProt ID O70274
Cytogenetics 4 D2.2
MW 19.1 kDa
Summary Protein tyrosine phosphatase which stimulates progression from G1 into S phase during mitosis. Inhibits geranylgeranyl transferase type II activity by blocking the association between RABGGTA and RABGGTB (By similarity).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008974" in other vectors (4)
SKU Description Size Price
MC207368 Ptp4a2 (untagged) - Mouse protein tyrosine phosphatase 4a2 (Ptp4a2), transcript variant 1, (10ug) 10 ug
$330.00
MG201376 Ptp4a2 (tGFP-tagged) - Mouse protein tyrosine phosphatase 4a2 (Ptp4a2) 10 ug
$500.00
MR201376L3 Lenti ORF clone of Ptp4a2 (Myc-DDK-tagged) - Mouse protein tyrosine phosphatase 4a2 (Ptp4a2), transcript variant 1 10 ug
$600.00
MR201376L4 Lenti ORF clone of Ptp4a2 (mGFP-tagged) - Mouse protein tyrosine phosphatase 4a2 (Ptp4a2), transcript variant 1 10 ug
$600.00

Plasmid Map for MR201376

Download sequence for

Cloning scheme for MR201376

Circular map for MR201376

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