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Ufc1 (NM_025388) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201362
Ufc1 (Myc-DDK-tagged) - Mouse ubiquitin-fold modifier conjugating enzyme 1 (Ufc1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ufc1 or NM_025388"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ufc1
Synonyms 1110021H02Rik; AA062310; ESTM29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201362 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGAGGCCACTCGGCGGGTCGTGTCTGAGATCCCGGTGCTGAAGACCAATGCCGGACCCCGAG
ATCGGGAATTGTGGGTGCAGCGACTAAAGGAGGAATATCAGTCCCTGATCCGGTATGTGGAGAACAACAA
GAATTCGGACAATGATTGGTTCCGACTGGAGTCCAACAAGGAAGGGACCCGGTGGTTTGGAAAATGCTGG
TACATCCACGATTTCCTCAAATATGAGTTCGACATCGAGTTTGAAATTCCTATCACATATCCCACTACTG
CTCCGGAAATTGCAGTCCCTGAGCTGGACGGGAAGACAGCAAAGATGTACAGGGGTGGCAAAATATGTCT
GACTGATCATTTCAAACCTTTGTGGGCCAGGAATGTGCCTAAGTTTGGACTAGCTCATCTCATGGCCCTG
GGGCTGGGTCCTTGGCTGGCAGTGGAAGTCCCCGATCTGATCCAGAAGGGTGTGATCCAGCACAAAGAAA
AATGCAGCCAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201362 protein sequence
Red=Cloning site Green=Tags(s)

MADEATRRVVSEIPVLKTNAGPRDRELWVQRLKEEYQSLIRYVENNKNSDNDWFRLESNKEGTRWFGKCW
YIHDFLKYEFDIEFEIPITYPTTAPEIAVPELDGKTAKMYRGGKICLTDHFKPLWARNVPKFGLAHLMAL
GLGPWLAVEVPDLIQKGVIQHKEKCSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025388
ORF Size 501 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025388.1, NM_025388.2, NP_079664.1
RefSeq Size 964 bp
RefSeq ORF 504 bp
Locus ID 66155
UniProt ID Q9CR09
Cytogenetics 1 79.31 cM
MW 19.5 kDa
Summary E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage.UniProtKB/Swiss-Prot Function
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"NM_025388" in other vectors (4)
SKU Description Size Price
MC204187 Ufc1 (untagged) - Mouse ubiquitin-fold modifier conjugating enzyme 1 (Ufc1), (10ug) 10 ug
$300.00
MG201362 Ufc1 (tGFP-tagged) - Mouse ubiquitin-fold modifier conjugating enzyme 1 (Ufc1) 10 ug
$500.00
MR201362L3 Lenti ORF clone of Ufc1 (Myc-DDK-tagged) - Mouse ubiquitin-fold modifier conjugating enzyme 1 (Ufc1) 10 ug
$600.00
MR201362L4 Lenti ORF clone of Ufc1 (mGFP-tagged) - Mouse ubiquitin-fold modifier conjugating enzyme 1 (Ufc1) 10 ug
$600.00

Plasmid Map for MR201362

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201362
Circular map for MR201362

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