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Cep19 (NM_025892) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201297
Cep19 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cep19
Synonyms 1500031L02Rik; AI428934; AL022620
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201297 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATACATTGCCAAGAAATGTGGAGTTAGGTTCCAGCCTCCAGCTGTGATCTTGATTTATGAGAATG
AAACCGAAGGAAAGAGCCGCCAGCGTATCATGCCTGTCCGAAACTTTTCAAAGTTCTCAGATTGCACCAG
AGCTGCGGAACAGTTAAAGAATAACCCACGGCACAAGAGTTACCTGGAACAGGTGCCCCTGAAGCAGCTG
GAGAAGCTGTTTGTTTTTTTGCGAGGTTCCTTGCAGGGGCAGAGCTTGGCAGAAACAATGGAACAGATTC
GGCGGGAAACGACCATCGATCCCGAGGAAGACCTGAACAAACTGGACGACAAGGAGCTCGCCAAAAGGAA
GAGCATCATGGATGAGCTTTTCGAGAAAAATCAGAAGAGAAAGGACGACCCCACTTTTGTGTACGACGTC
GAGGTGGAGTTCCCTCAGGATGAACAGCTGCTGTCCTGCAGCTGGGACACAGCGTCAGTGGATGACTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201297 protein sequence
Red=Cloning site Green=Tags(s)

MKYIAKKCGVRFQPPAVILIYENETEGKSRQRIMPVRNFSKFSDCTRAAEQLKNNPRHKSYLEQVPLKQL
EKLFVFLRGSLQGQSLAETMEQIRRETTIDPEEDLNKLDDKELAKRKSIMDELFEKNQKRKDDPTFVYDV
EVEFPQDEQLLSCSWDTASVDDF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025892
ORF Size 489 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025892.3
RefSeq Size 1701 bp
RefSeq ORF 492 bp
Locus ID 66994
UniProt ID Q9CQA8
Cytogenetics 16 B2
MW 19.2 kDa
Summary Required for ciliation. Recruits the RABL2B GTPase to the ciliary base to initiate ciliation. After specifically capturing the activated GTP-bound RABL2B, the CEP19-RABL2B complex binds intraflagellar transport (IFT) complex B from the large pool pre-docked at the base of the cilium and thus triggers its entry into the cilia. Involved in the early steps in cilia formation by recruiting the ciliary vesicles (CVs) to the distal end of the mother centriole where they fuse to initiate cilium assembly. Involved in microtubule (MT) anchoring at centrosomes.UniProtKB/Swiss-Prot Function
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"NM_025892" in other vectors (4)
SKU Description Size Price
MC207603 Cep19 (untagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik), (10ug) 10 ug
$165.00
MG201297 Cep19 (tGFP-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik) 10 ug
$350.00
MR201297L3 Lenti ORF clone of Cep19 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik) 10 ug
$450.00
MR201297L4 Lenti ORF clone of Cep19 (mGFP-tagged) - Mouse RIKEN cDNA 1500031L02 gene (1500031L02Rik) 10 ug
$450.00

Plasmid Map for MR201297

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for MR201297
Circular map for MR201297

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