Mphosph6 (NM_026758) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201256
Mphosph6 (Myc-DDK-tagged) - Mouse M phase phosphoprotein 6 (Mphosph6)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mphosph6
Synonyms 1110001M01Rik; AA536809; C86426
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201256 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCGGAGCGCAAGACTAAGTTATCCAAGAACCTGCTGCGCATGAAGTTCATGCAAAGAGGCCTGG
ACTCGGAAACCAAGAAACAGCTAGAAGAGGAGGAGCGGAAGATGATCAGCGACGAGCACTGGTACCTGGA
TTTGCCGGAGTTGAAGGAGAAGGAGAGCTTCATCGTAGAGGAGCAGAGCTTCTCCCTGTGTGAAGATCTT
CTCTACGGAAGGATGTCATTCAGGGGGTTTAATCCTGAGGTTGAGAAGTTAATGCTTCAGATGAATTCTA
AGAACCGAGCAGAGGCAGCAGAGGAAGACGAGACGGTAGAGGTCGATGTGTCTGATGAGGAAATGGCAAG
AAGATATGAGACTTTGGTGGGAACAATTGGAAAAAAGTTTGTCAAGAAAAGGGACCGAGCCAATTACGAA
GAAGATGAAAATGGAACTATAAAAGCCATAAAACCTAAGAAAATGTTCTTAAAGCCCCAAGAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201256 protein sequence
Red=Cloning site Green=Tags(s)

MASERKTKLSKNLLRMKFMQRGLDSETKKQLEEEERKMISDEHWYLDLPELKEKESFIVEEQSFSLCEDL
LYGRMSFRGFNPEVEKLMLQMNSKNRAEAAEEDETVEVDVSDEEMARRYETLVGTIGKKFVKKRDRANYE
EDENGTIKAIKPKKMFLKPQD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026758
ORF Size 483 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026758.4
RefSeq Size 1054 bp
RefSeq ORF 486 bp
Locus ID 68533
UniProt ID Q9D1Q1
Cytogenetics 8 E1
MW 19.1 kDa
Summary RNA-binding protein that associates with the RNA exosome complex. Involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and plays a role in recruiting the RNA exosome complex to pre-rRNA; this function may include C1D.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_026758" in other vectors (3)
SKU Description Size Price
MC200775 Mphosph6 (untagged) - Mouse M phase phosphoprotein 6 (Mphosph6), (10ug) 10 ug
$150.00
MR201256L3 Lenti ORF clone of Mphosph6 (Myc-DDK-tagged) - Mouse M phase phosphoprotein 6 (Mphosph6) 10 ug
$450.00
MR201256L4 Lenti ORF clone of Mphosph6 (mGFP-tagged) - Mouse M phase phosphoprotein 6 (Mphosph6) 10 ug
$450.00

Plasmid Map for MR201256

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Cloning scheme for MR201256

Circular map for MR201256

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