Ormdl2 (NM_024180) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR201119
Ormdl2 (Myc-DDK-tagged) - Mouse ORM1-like 2 (S. cerevisiae) (Ormdl2)
  $289.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ormdl2
Synonyms 0610012C09Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201119 representing NM_024180
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCCGTCCTCGGTGCCCCATCCGCTCCCGCCGCCAGGGCAGCAAGTCATCCACGTCACGCAGGACC
TGGACACCGACCTCGAAGCCCTCTTCAACTCTGTCATGAACCCCAAGCCCAGCTCATGGCGGAAAAAGAT
CCTCCCGGAGTCCTTCTTTAAGGAGCCCGATTCCGGCTCGCACTCGCGCCAATCCAGCACAGACTCATCA
GGCGGCCACCCGGGGCCTCGACTAGCTGGCGGCGCGCAGCACGTCCGCTCGCACTCGTCGCCCGCATCCC
TGCAGCTGGGCACCGGTGCGGGAGCCGCTGGAGGCCCTGCACAGCAGCATGCACATCTCCGCCAGCAGTC
CTATGACGTGACCGACGAGCTGCCGTTGCCCCCCGGGTGGGAGATGACCTTCACGGCCACTGGCCAGAGA
TACTTCCTTAATCACATAGAGAAAATCACCACATGGCAAGACCCCAGGAAGGTGATGAATCAGCCTCTGA
ATCATGTGAACCTCCACCCGTCCATCACTTCCACCTCGGTGCCACAGAGGTCCATGGCAGTGTCCCAGCC
GAATCTCGCAATGAATCACCAACACCAGCAAGTCGTGGCCACTAGCCTGAGTCCACAGAACCACCCGACT
CAGAACCAACCCACAGGGCTCATGAGTGTGCCCAATGCACTGACCACTCAGCAGCAGCAGCAGCAGAAAC
TGCGGCTTCAGAGGATCCAGATGGAGAGAGAGAGGATTAGGATGCGTCAAGAGGAGCTCATGAGGCAGGA
AGCTGCCCTCTGCCGACAGCTCCCCATGGAAACCGAGACCATGGCCCCTGTCAACACGCCTGCCATGAGC
ACAGATATGAGATCTGTCACCAACAGTAGCTCAGATCCTTTCCTCAATGGAGGGCCCTATCATTCACGGG
AGCAGAGCACAGACAGTGGCCTGGGGTTAGGGTGCTACAGTGTCCCCACAACTCCAGAAGACTTCCTCAG
CAACATGGACGAGATGGATACAGGTGAAAATTCCGGTCAGACACCCATGACCGTCAATCCCCAGCAGACC
CGCTTCCCTGATTTCCTGGACTGCCTTCCAGGAACAAATGTTGACCTCGGGACTTTGGAGTCTGAAGATC
TGATCCCTCTCTTCAATGATGTAGAGTCTGCTCTGAACAAAAGCGAGCCCTTTCTAACCTGGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR201119 representing NM_024180
Red=Cloning site Green=Tags(s)

MNPSSVPHPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSS
GGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGGPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQR
YFLNHIEKITTWQDPRKVMNQPLNHVNLHPSITSTSVPQRSMAVSQPNLAMNHQHQQVVATSLSPQNHPT
QNQPTGLMSVPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMETETMAPVNTPAMS
TDMRSVTNSSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNMDEMDTGENSGQTPMTVNPQQT
RFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_024180
ORF Size 459 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_024180.6
RefSeq Size 2055 bp
RefSeq ORF 462 bp
Locus ID 66844
UniProt ID Q9CQZ0
Cytogenetics 10 D3
MW 17.4 kDa
Summary Negative regulator of sphingolipid synthesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_024180" in other vectors (4)
SKU Description Size Price
MC200317 Ormdl2 (untagged) - Mouse ORM1-like 2 (S. cerevisiae) (Ormdl2), (10ug) 10 ug
$150.00
MG201119 Ormdl2 (tGFP-tagged) - Mouse ORM1-like 2 (S. cerevisiae) (Ormdl2) 10 ug
$350.00
MR201119L3 Lenti ORF clone of Ormdl2 (Myc-DDK-tagged) - Mouse ORM1-like 2 (S. cerevisiae) (Ormdl2) 10 ug
$450.00
MR201119L4 Lenti ORF clone of Ormdl2 (mGFP-tagged) - Mouse ORM1-like 2 (S. cerevisiae) (Ormdl2) 10 ug
$450.00

Plasmid Map for MR201119

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Cloning scheme for MR201119

Circular map for MR201119

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