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Arpc5 (NM_026369) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201085
Arpc5 (Myc-DDK-tagged) - Mouse actin related protein 2/3 complex, subunit 5 (Arpc5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "Arpc5 or NM_026369"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Arpc5
Synonyms 5830443F10Rik; p16-Arc
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201085 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAAGAACACGGTGTCGTCGGCCCGCTTCCGGAAGGTGGACGTGGACGAATATGACGAGAACAAGT
TCGTGGACGAGGAGGACGGCGGCGATGGCCAGGCCGGGCCCGACGAGGGCGAGGTGGACTCGTGCCTACG
GCAAGGAAACATGACAGCTGCTCTCCAGGCTGCTCTGAAAAACCCCCCTATTAACACAAAGAGCCAGGCA
GTGAAGGACCGTGCAGGCAGCATCGTCTTGAAGGTGCTTATCTCTTTTAAAGCTAATGATATTGAAAAGG
CAGTGCAGTCTCTGGACAAGAACGGTGTGGACCTCCTAATGAAGTATATTTATAAAGGCTTTGAGAGCCC
ATCTGACAACAGCAGTGCTGTGTTGCTCCAGTGGCATGAAAAGGCCCTTGCTGCTGGAGGAGTGGGGTCC
ATTGTTCGAGTTTTGACTGCAAGGAAAACCGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201085 protein sequence
Red=Cloning site Green=Tags(s)

MSKNTVSSARFRKVDVDEYDENKFVDEEDGGDGQAGPDEGEVDSCLRQGNMTAALQAALKNPPINTKSQA
VKDRAGSIVLKVLISFKANDIEKAVQSLDKNGVDLLMKYIYKGFESPSDNSSAVLLQWHEKALAAGGVGS
IVRVLTARKTV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026369
ORF Size 453 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026369.2
RefSeq Size 1897 bp
RefSeq ORF 456 bp
Locus ID 67771
UniProt ID Q9CPW4
Cytogenetics 1 G3
MW 16.3 kDa
Summary Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).UniProtKB/Swiss-Prot Function
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"NM_026369" in other vectors (4)
SKU Description Size Price
MC202579 Arpc5 (untagged) - Mouse actin related protein 2/3 complex, subunit 5 (Arpc5), (10ug) 10 ug
$150.00
MG201085 Arpc5 (tGFP-tagged) - Mouse actin related protein 2/3 complex, subunit 5 (Arpc5) 10 ug
$350.00
MR201085L3 Lenti ORF clone of Arpc5 (Myc-DDK-tagged) - Mouse actin related protein 2/3 complex, subunit 5 (Arpc5) 10 ug
$450.00
MR201085L4 Lenti ORF clone of Arpc5 (mGFP-tagged) - Mouse actin related protein 2/3 complex, subunit 5 (Arpc5) 10 ug
$450.00

Plasmid Map for MR201085

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201085
Circular map for MR201085

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