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Polr2h (BC092087) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201055
Polr2h (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Polr2h or BC092087"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Polr2h
Synonyms MGC8248
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201055 representing BC092087
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGCATCCTGTTCGAGGATATTTTCGATGTGAAAGACATCGACCCAGAAGGCAAGAAATTTGACC
GAGTATCCCGGCTGCACTGTGAGAGTGAATCTTTCAAGATGGACCTCATCTTAGATGTAAACATTCAGAT
TTACCCTGTGGACTTAGGTGACAAGTTCCGGTTGGTTATAGCTAGTACTTTGTATGAAGATGGTACCTTG
GATGATGGTGAATACAACCCCACAGATGACAGGCCTTCCAGGGCTGACCAGTTTGAGTATGTCATGTATG
GGAAAGTGTACAGGATCGAAGGGGATGAGACCTCCACTGAAGCAGCCACACGCCTCTCTGCGTACGTGTC
CTATGGGGGCCTGCTCATGAGGCTTCAGGGTGATGCCAACAACCTGCATGGATTTGAGGTGGACTCCAGA
GTTTATCTGCTGATGAAGAAGCTGGCCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201055 representing BC092087
Red=Cloning site Green=Tags(s)

MAGILFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTLYEDGTL
DDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMRLQGDANNLHGFEVDSR
VYLLMKKLAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN BC092087
ORF Size 450 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC092087.1
RefSeq Size 758 bp
RefSeq ORF 452 bp
Locus ID 245841
Cytogenetics 16 B1
MW 17.1 kDa
Summary DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively (By similarity).UniProtKB/Swiss-Prot Function
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"BC092087" in other vectors (3)
SKU Description Size Price
MG201055 Polr2h (tGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770) 10 ug
$350.00
MR201055L3 Lenti ORF clone of Polr2h (Myc-DDK-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770) 10 ug
$450.00
MR201055L4 Lenti ORF clone of Polr2h (mGFP-tagged) - Mouse polymerase (RNA) II (DNA directed) polypeptide H (cDNA clone MGC:103049 IMAGE:5042770) 10 ug
$450.00

Plasmid Map for MR201055

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201055
Circular map for MR201055

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