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Cst6 (NM_028623) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR201045
Cst6 (Myc-DDK-tagged) - Mouse cystatin E/M (Cst6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cst6
Synonyms 1110017E11Rik; ichq; N28197
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR201045 representing NM_028623
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCGTCCTCACTTCCCGCTGGCGATGGGCCTTGGCCTGCTCGCATTCTGCCTCCTGACCCTGTCAC
CTGACGCCCGCGCCGAGCTGCGGAGCCGCAGGACCGGAGAACGCCAGAACTTGTCACCCACCGACCCGCG
GGTGCAAAAGGCCGCACAGGCAGCTGTGGCCAGCTACAATATGGGCAGCGACAGCCTCTACTACTTCCGA
GACACCAAAGTCATCGATGCAAAATATCAGCTGGTGGCTGGCATCAAGTACTACCTGACTTTGGACATAG
AAAGCACAGAGTGCCGAAAGACCAGAGTCTCTGGAGAACACATGGACCTCACCACCTGCCCTCTGGCTGC
AGGGGGGCAGCAGGAGAAACTGCGTTGCAACTTTGAGCTCCTTGAAGTCCCCTGGAAGAACACTACTCAG
CTTCTAAAGCATGACTGCGTGCAGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR201045 representing NM_028623
Red=Cloning site Green=Tags(s)

MERPHFPLAMGLGLLAFCLLTLSPDARAELRSRRTGERQNLSPTDPRVQKAAQAAVASYNMGSDSLYYFR
DTKVIDAKYQLVAGIKYYLTLDIESTECRKTRVSGEHMDLTTCPLAAGGQQEKLRCNFELLEVPWKNTTQ
LLKHDCVQV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028623
ORF Size 447 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028623.5, NP_082899.1
RefSeq Size 3951 bp
RefSeq ORF 450 bp
Locus ID 73720
Cytogenetics 19 4.3 cM
MW 17.2 kDa
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"NM_028623" in other vectors (4)
SKU Description Size Price
MC204949 Cst6 (untagged) - Mouse cystatin E/M (Cst6), (10ug) 10 ug
$150.00
MG201045 Cst6 (tGFP-tagged) - Mouse cystatin E/M (Cst6) 10 ug
$350.00
MR201045L3 Lenti ORF clone of Cst6 (Myc-DDK-tagged) - Mouse cystatin E/M (Cst6) 10 ug
$450.00
MR201045L4 Lenti ORF clone of Cst6 (mGFP-tagged) - Mouse cystatin E/M (Cst6) 10 ug
$450.00

Plasmid Map for MR201045

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR201045
Circular map for MR201045

file-alt Citations

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