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Rps15 (NM_009091) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200959
Rps15 (Myc-DDK-tagged) - Mouse ribosomal protein S15 (Rps15)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rps15
Synonyms rig
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200959 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGAAGTGGAGCAGAAGAAGAAGCGCACCTTCCGCAAGTTCACCTACCGTGGCGTAGACCTCGACC
AACTGCTCGACATGTCCTATGAGCAACTGATGCAGCTGTACAGCGCCCGCCAGAGGAGGCGCCTGAACCG
TGGTCTTCGGCGGAAGCAACACTCACTGCTCAAGCGCTTGAGAAAGGCCAAAAAGGAGGCACCACCCATG
GAGAAGCCTGAGGTGGTGAAGACTCACCTGAGGGACATGATCATCCTGCCGGAGATGGTGGGTAGCATGG
TGGGCGTGTACAACGGCAAGACCTTCAACCAGGTGGAGATCAAACCAGAGATGATCGGCCACTACCTGGG
CGAGTTCTCCATCACCTACAAACCCGTGAAGCACGGCCGGCCCGGGATCGGTGCCACCCACTCCTCCCGA
TTCATCCCCCTCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200959 protein sequence
Red=Cloning site Green=Tags(s)

MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLYSARQRRRLNRGLRRKQHSLLKRLRKAKKEAPPM
EKPEVVKTHLRDMIILPEMVGSMVGVYNGKTFNQVEIKPEMIGHYLGEFSITYKPVKHGRPGIGATHSSR
FIPLK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009091
ORF Size 435 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009091.1, NP_033117.1
RefSeq Size 522 bp
RefSeq ORF 438 bp
Locus ID 20054
UniProt ID P62843
Cytogenetics 10 C1
MW 17 kDa
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Documents
Resources
"NM_009091" in other vectors (4)
SKU Description Size Price
MC203833 Rps15 (untagged) - Mouse ribosomal protein S15 (Rps15), (10ug) 10 ug
$150.00
MG200959 Rps15 (tGFP-tagged) - Mouse ribosomal protein S15 (Rps15) 10 ug
$350.00
MR200959L3 Lenti ORF clone of Rps15 (Myc-DDK-tagged) - Mouse ribosomal protein S15 (Rps15) 10 ug
$450.00
MR200959L4 Lenti ORF clone of Rps15 (mGFP-tagged) - Mouse ribosomal protein S15 (Rps15) 10 ug
$450.00

Plasmid Map for MR200959

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200959
Circular map for MR200959

file-alt Citations

The Diabetes Gene JAZF1 Is Essential for the Homeostatic Control of Ribosome Biogenesis and Function in Metabolic Stress
Kobiita, Godbersen, Araldi et al
Cell Rep (2020) 32 (1), 107846 DOI: 10.1016/j.celrep.2020.107846
Cited by 59
AI Summary: In this study, OriGene’s Myc-DDK-tagged expression plasmids for Nop56, Tsr3, Rps2, Rps11, Rps14, Rps15, Rps20, Rpl10a, Rpl12, Rpl13, and Rpl13a were used to investigate the role of specific ribosomal proteins and factors in elucidating how JAZF1 regulates ribosome biogenesis and function in pancreatic β-cells under metabolic stress.
Snippet: Jazf1 adenovirus: Ad: Jazf1-HA-tagged This paper N/A Gfp adenovirus: Ad:GFP This paper N/A Nop56 (Myc-DDK-tagged) Origene MR209091 Tsr3 (Myc-DDK-tagged) Origene MR204683 Rps2 (Myc-DDK-tagged) Origene MR204027 Rps11 (Myc-DDK-tagged) Origene MR216516 Rps14 (Myc-DDK-tagged) Origene RC203889 Rps15 (Myc-DDK-tagged) Origene MR200959 Rps20 (Myc-DDK-tagged) Origene MR200561 Rpl10a (Myc-DDK-tagged) Origene MR200782 Rpl12 (Myc-DDK-tagged) Origene MR201323 Rpl13 (Myc-DDK-tagged) Origene MR202263 Rpl13a (Myc-DDK-tagged) Origene MR202103
Showing 1-1 of 1 papers.
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