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Lypd1 (NM_145100) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200904
Lypd1 (Myc-DDK-tagged) - Mouse Ly6/Plaur domain containing 1 (Lypd1)
Interest in protein/lysate? Submit request here!
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Lypd1 or NM_145100"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Lypd1
Synonyms 2700050C12Rik; AI853408; C530008O16Rik; Lynx2; Lypdc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200904 representing NM_145100
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGTTCTCGGCATCGCAGCAACTTTTTGCGGATTGTTCTGGCTTCCAGGGCTGGCGCTGCAAATTC
AGTGCTACCAGTGTGAAGAATTCCAGCTGAACAACGATTGCTCATCCCCTGAGTTCATCGTAAATTGCAC
CGTGAACGTTCAAGACATGTGTCAGAAAGAAGTGATGGAGCAAAGTGCTGGGATCATGTACCGGAAGTCG
TGTGCATCGTCAGCAGCCTGTCTCATTGCTTCAGCTGGGTACCAGTCCTTCTGTTCCCCTGGGAAACTGA
ACTCCGTGTGCATCAGCTGCTGCAACACCCCTCTTTGCAATGGGCCGAGGCCCAAGAAGAGAGGCAGCTC
TGCCTCGGCCATCAGGCCAGGGCTTCTCACCACTCTCCTGTTCTTCCACTTAGCCCTCTGCTTGGCACAC
TGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200904 representing NM_145100
Red=Cloning site Green=Tags(s)

MWVLGIAATFCGLFWLPGLALQIQCYQCEEFQLNNDCSSPEFIVNCTVNVQDMCQKEVMEQSAGIMYRKS
CASSAACLIASAGYQSFCSPGKLNSVCISCCNTPLCNGPRPKKRGSSASAIRPGLLTTLLFFHLALCLAH
C

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145100
ORF Size 423 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145100.4, NP_659568.2
RefSeq Size 2047 bp
RefSeq ORF 426 bp
Locus ID 72585
UniProt ID Q8BLC3
Cytogenetics 1 E3
MW 15.7 kDa
Summary Believed to act as a modulator of nicotinic acetylcholine receptors (nAChRs) activity. In vitro increases receptor desensitization and decreases affinity for ACh of alpha-4:beta-2-containing nAChRs (PubMed:19246390). May play a role in the intracellular trafficking of alpha-4:beta-2 and alpha-7-containing nAChRs and may inhibit their expression at the cell surface (PubMed:26276394, PubMed:25716842). May be involved in the control of anxiety (PubMed:19246390).UniProtKB/Swiss-Prot Function
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Documents
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"NM_145100" in other vectors (3)
SKU Description Size Price
MC211344 Lypd1 (untagged) - Mouse Ly6/Plaur domain containing 1 (Lypd1), (10ug) 10 ug
$165.00
MR200904L3 Lenti ORF clone of Lypd1 (Myc-DDK-tagged) - Mouse Ly6/Plaur domain containing 1 (Lypd1) 10 ug
$450.00
MR200904L4 Lenti ORF clone of Lypd1 (mGFP-tagged) - Mouse Ly6/Plaur domain containing 1 (Lypd1) 10 ug
$450.00

Plasmid Map for MR200904

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200904
Circular map for MR200904

file-alt Citations

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