Ap2s1 (NM_198613) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR200768
Ap2s1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 2, sigma 1 subunit (Ap2s1)
$289.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Ap2s1 |
| Synonyms | AI043088 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_198613 |
| ORF Size | 396 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_198613.2 |
| RefSeq Size | 798 bp |
| RefSeq ORF | 429 bp |
| Locus ID | 232910 |
| UniProt ID | P62743 |
| Cytogenetics | 7 A2 |
| MW | 15.9 kDa |
| Summary | Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-FILMV (Y-X-X-Phi) and ED-X-X-X-L-LI endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the ED-X-X-X-L-LI motif. May also play a role in extracellular calcium homeostasis (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_198613" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG200768 | Ap2s1 (tGFP-tagged) - Mouse adaptor-related protein complex 2, sigma 1 subunit (Ap2s1) | 10 ug |
$350.00
|
|
| MR200768L3 | Lenti ORF clone of Ap2s1 (Myc-DDK-tagged) - Mouse adaptor-related protein complex 2, sigma 1 subunit (Ap2s1) | 10 ug |
$450.00
|
|
| MR200768L4 | Lenti ORF clone of Ap2s1 (mGFP-tagged) - Mouse adaptor-related protein complex 2, sigma 1 subunit (Ap2s1) | 10 ug |
$450.00
|
|
Citations
ASTN2 modulates synaptic strength by trafficking and degradation of surface proteins
Behesti, Fore, Wu et al
Proc Natl Acad Sci U S A (2018) 115 (41), E9717-E9726 DOI: 10.1073/pnas.1809382115
Behesti, Fore, Wu et al
Proc Natl Acad Sci U S A (2018) 115 (41), E9717-E9726 DOI: 10.1073/pnas.1809382115
Cited by 67
AI Summary: OriGene products, specifically the pCMV6-AN-Myc and pCMV6-Entry expression plasmids, were used in this study to overexpress ASTN2 and its variants in neurons, enabling the investigation of ASTN2's role in regulating synaptic protein surface expression and synaptic activity.
ASTN2 modulates synaptic strength by trafficking and degradation of surface proteins
Behesti, Fore, Wu et al
bioRxiv (2018)
Behesti, Fore, Wu et al
bioRxiv (2018)
Snippet: &The,following,plasmids,were,used:,pGIPZ,lentiviral, plasmids,containing,Astn2,shRNA,(#,V3LMM440320),and,scramble,shRNA,(Thermo, Scientific,Open,Biosystems),,OLFM15MYC5Flag,(Origene,#MR207779),,AP2s5MYC5 Flag,(Origene,#MR200768),,Clqc5MYC5Flag,(Origene,#MR203092),,pCAG:GPI5GFP, (Addgene,#32601),,pMES5SLC12a55HA,(50),,pNice5NLGN15CFP,,pNice5NLGN25CFP
Showing 1-2 of 2 papers.
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