Cxcl5 (NM_009141) Mouse Tagged ORF Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Cxcl5 |
| Synonyms | AMCF; AMCF-II; Cxcl; Cxcl6; ENA-; ENA-78; GCP-; GCP-2; L; LIX; Scyb; Scyb5; Scyb6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_009141 |
| ORF Size | 396 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_009141.1 |
| RefSeq Size | 1655 bp |
| RefSeq ORF | 399 bp |
| Locus ID | 20311 |
| UniProt ID | P50228 |
| Cytogenetics | 5 44.78 cM |
| MW | 14.7 kDa |
| Summary | This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils and to have homeostatic and inflammatory functions. In mouse, deficiency of this gene is associated with increased lung inflammation that is neutrophil-dependent. provided by RefSeq, May 2013 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009141" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC202149 | Cxcl5 (untagged) - Mouse chemokine (C-X-C motif) ligand 5 (Cxcl5), (10ug) | 10 ug |
$225.00
|
|
| MG200761 | Cxcl5 (tGFP-tagged) - Mouse chemokine (C-X-C motif) ligand 5 (Cxcl5) | 10 ug |
$425.00
|
|
| MR200761L3 | Lenti ORF clone of Cxcl5 (Myc-DDK-tagged) - Mouse chemokine (C-X-C motif) ligand 5 (Cxcl5) | 10 ug |
$525.00
|
|
| MR200761L4 | Lenti ORF clone of Cxcl5 (mGFP-tagged) - Mouse chemokine (C-X-C motif) ligand 5 (Cxcl5) | 10 ug |
$525.00
|
|
Citations
IL-17/CXCL5 signaling within the oligovascular niche mediates human and mouse white matter injury
Xiao, Kumar, Komuro et al
Cell Rep (2022) 41 (12), 111848 DOI: 10.1016/j.celrep.2022.111848
Xiao, Kumar, Komuro et al
Cell Rep (2022) 41 (12), 111848 DOI: 10.1016/j.celrep.2022.111848
Cited by 17
AI Summary: OriGene's murine CXCL5 plasmid (#MR200761) was used to overexpress CXCL5 in experimental models, enabling the study of its role in white matter injury and oligovascular signaling pathways.
Snippet: fiber diameter, axon diameter, myelin thickness, and g-ratio. Lentiviral injection A plasmid containing the open reading frame of the murine CXCL5 sequence with a 3′ stop codon was purchased from Origene (#MR200761). The pCDH-EF1-FLEX-EGFP-CMV-2A-TagBFP2-SC dual promoter lentiviral backbone was created by subcloning the FLEX-GFP sequence between the loxP sites from the pAAV-FLEX-GFP vector (Ad
Endothelial CXCL5 negatively regulates myelination and repair after white matter stroke
Xiao, Kumar, Burguet et al
bioRxiv (2019)
Xiao, Kumar, Burguet et al
bioRxiv (2019)
Snippet: A plasmid containing the open reading frame of the murine CXCL5 sequence with a 3’ stop codon was purchased from Origene (#MR200761). The pCDH-EF1-FLEX-EGFP-CMV2A-TagBFP2-SC dual promoter lentiviral backbone was created by subcloning the FLEX-GFP sequence between the loxP sites from the pAAV-FLEX-GFP vector (Addgene #28304) into the
Showing 1-2 of 2 papers.
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