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Gemin7 (NM_027189) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200714
Gemin7 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 7 (Gemin7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Gemin7 or NM_027189"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gemin7
Synonyms 2400008I04Rik; AI120175; AI461688
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200714 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGAGCCCACTGACCATTCCTGTGCCGGTGCCAGTGCTGCGCCTCCCTCGGGGCCCCGATGGCTTCA
GCAGAGGCTTTGCCTCTGATGGACGTAGGACCATCCTGAGACCAGAAGTCGGAGAAGGTCATATTCAAGA
TCCCCCAGAGTCACAGGAACAGCGGGCCCGAGCCACTCTGCGAGAGCGCTACCTCCGCAGCCTCCTAGCC
ATGGTGGGGCACCCTGTGAGTTTCACGCTGCACGAAGGTGTGCATGTGACCGCCCAGTTTGGAGCCACAG
ACCTGGATGTGGCCAACTTCTACGTTTCCCAACTGCAGACTCCCATAGGTGTACAGGCCGAGGCCTTGCT
TCGATGTAGTGACATAATTTCCTATTCTTTCAAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200714 protein sequence
Red=Cloning site Green=Tags(s)

MQSPLTIPVPVPVLRLPRGPDGFSRGFASDGRRTILRPEVGEGHIQDPPESQEQRARATLRERYLRSLLA
MVGHPVSFTLHEGVHVTAQFGATDLDVANFYVSQLQTPIGVQAEALLRCSDIISYSFKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027189
ORF Size 387 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027189.2
RefSeq Size 763 bp
RefSeq ORF 390 bp
Locus ID 69731
UniProt ID Q9CWY4
Cytogenetics 7 A3
MW 14.3 kDa
Summary The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome. Thereby, plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP. In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. Dissociation by the SMN complex of CLNS1A from the trapped Sm proteins and their transfer to an SMN-Sm complex triggers the assembly of core snRNPs and their transport to the nucleus (By similarity).UniProtKB/Swiss-Prot Function
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"NM_027189" in other vectors (4)
SKU Description Size Price
MC201365 Gemin7 (untagged) - Mouse gem (nuclear organelle) associated protein 7 (Gemin7), (10ug) 10 ug
$150.00
MG200714 Gemin7 (tGFP-tagged) - Mouse gem (nuclear organelle) associated protein 7 (Gemin7) 10 ug
$350.00
MR200714L3 Lenti ORF clone of Gemin7 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 7 (Gemin7) 10 ug
$450.00
MR200714L4 Lenti ORF clone of Gemin7 (mGFP-tagged) - Mouse gem (nuclear organelle) associated protein 7 (Gemin7) 10 ug
$450.00

Plasmid Map for MR200714

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200714
Circular map for MR200714

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