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Sf3b6 (NM_025323) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR200641
0610009D07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Sf3b6 or NM_025323"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sf3b6
Synonyms 0610009D07Rik; 6030419K15Rik; AV001342; Sf3b14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR200641 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGATGCAAGCGGCTAAGAGGGCGAATATTCGCCTCCCACCTGAAGTAAATCGGATTTTGTATATAA
GGAATTTGCCATACAAAATCACAGCAGAAGAAATGTATGATATATTTGGGAAATATGGACCTATTCGTCA
AATCAGAGTAGGGAATACACCTGAAACCAGAGGAACAGCCTATGTGGTCTATGAGGACATCTTTGATGCT
AAGAATGCCTGCGACCACCTATCAGGATTCAACGTTTGTAACAGATACCTTGTGGTTTTGTATTACAATG
CTAACCGGGCATTTCAGAAGATGGACACCAAGAAGAAGGAGGAGCAGCTGAAACTTCTCAAGGAAAAGTA
TGGCATCAACACAGATCCTCCCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR200641 protein sequence
Red=Cloning site Green=Tags(s)

MAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDA
KNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_025323
ORF Size 375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_025323.2, NP_079599.1
RefSeq Size 1133 bp
RefSeq ORF 378 bp
Locus ID 66055
UniProt ID P59708
Cytogenetics 12 A1.1
MW 14.6 kDa
Summary Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.UniProtKB/Swiss-Prot Function
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"NM_025323" in other vectors (4)
SKU Description Size Price
MC203917 0610009D07Rik (untagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik), (10ug) 10 ug
$150.00
MG200641 0610009D07Rik (tGFP-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik) 10 ug
$350.00
MR200641L3 Lenti ORF clone of 0610009D07Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik) 10 ug
$450.00
MR200641L4 Lenti ORF clone of 0610009D07Rik (mGFP-tagged) - Mouse RIKEN cDNA 0610009D07 gene (0610009D07Rik) 10 ug
$450.00

Plasmid Map for MR200641

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR200641
Circular map for MR200641

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